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Product Info Summary
| SKU: | EK1307 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine®
View all TGF-beta RII ELISA kits
SKU/Catalog Number
EK1307
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TGFBR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1307)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: I24-D159
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1307 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1307 is reactive to TGFBR2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1307 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGF-beta RII
TGFBR2 (transforming growth factor, beta receptor II (70/80kDa)), also known as TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II ( TGF-beta receptor type II, TbetaR-II), is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. A TGFBR2 cDNA encoding a deduced 565-amino acid protein with a calculated molecular mass of approximately 60 kD in length. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different informs have been characterized. TGFBR2 may be a target of the EWS-FLI1 fusion protein found in Ewing sarcoma and related peripheral primitive neuroectodermal tumors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1307-CAP | Anti-Human TGFBR2 Pre-coated 96-well strip microplate | 1 |
| EK1307-ST | Human TGFBR2 Standard | 2 vials, 10 ng/tube |
| EK1307-DA | Human TGFBR2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.010 | 0.077 | 0.107 | 0.221 | 0.419 | 0.997 | 1.420 | 1.994 |
Data Example Images
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Human TGFBR2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TGF-beta RII, Dilution ratio of 1:4, concentration in serum and plasma is around 600 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 179 | 421 | 30 | 166 | 392 |
| Standard deviation | 2.34 | 7.7%3 | 18.1 | 2.31 | 8.3 | 21.16 |
| CV (%) | 7.1% | 4.6% | 4.3% | 7.7% | 5% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TGF-beta RII in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 34 | 33 | 36 | 34 | 1.22 | 3.5% |
| Sample 2 | 179 | 177 | 172 | 160 | 172 | 7.38 | 4.2% |
| Sample 3 | 421 | 443 | 453 | 438 | 438 | 11.58 | 2.6% |
Protein Target Info & Infographic
Gene/Protein Information For TGFBR2 (Source: Uniprot.Org, NCBI)
Gene Name
TGFBR2
Full Name
TGF-beta receptor type-2
Weight
64568 MW
Superfamily
protein kinase superfamily
Alternative Names
TGF-beta receptor type-2;TGFR-2;2.7.11.30;TGF-beta type II receptor;Transforming growth factor-beta receptor type II;TGF-beta receptor type II;TbetaR-II;TGFBR2; TGFBR2 AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII transforming growth factor beta receptor 2 TGF-beta receptor type-2|TGF-beta receptor type IIB|TGF-beta type II receptor|tbetaR-II|transforming growth factor beta receptor II|transforming growth factor beta receptor type IIC|transforming growth factor, beta receptor II (70/80kDa)|transforming growth factor, beta receptor II alpha|transforming growth factor, beta receptor II beta|transforming growth factor, beta receptor II delta|transforming growth factor, beta receptor II epsilon|transforming growth factor, beta receptor II gamma
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFBR2, check out the TGFBR2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFBR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine® (EK1307)
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Customer Q&As
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18 Customer Q&As for Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine®
Question
Can you advise whether the capture and detection antibodies host is monoclonal or polyclonal?
Verified customer
Asked: 2020-09-24
Answer
For the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307), the capture and detection antibodies are both goat polyclonal antibodies.
Boster Scientific Support
Answered: 2020-09-24
Question
What is the Expression host of the immunogen for the capture antibody of EK1307?
Verified customer
Asked: 2020-09-23
Answer
The Expression host of the immunogen for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is goat.
Boster Scientific Support
Answered: 2020-09-23
Question
Is the Immunogen for the capture antibody of EK1307 native or recombinant?
Verified customer
Asked: 2020-09-23
Answer
The Immunogen for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is recombinant.
Boster Scientific Support
Answered: 2020-09-23
Question
Is the Immunogen for the capture antibody of EK1307 full-length or partial?
Verified customer
Asked: 2020-09-23
Answer
The Immunogen for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is partial.
Boster Scientific Support
Answered: 2020-09-23
Question
What animal species would the immunogen sequence for the capture antibody of EK1307 comes from?
Verified customer
Asked: 2020-09-23
Answer
The animal species the immunogen sequence for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is Human.
Boster Scientific Support
Answered: 2020-09-23
Question
What animal species would the immunogen sequence for the detection antibody of EK1307 comes from?
Verified customer
Asked: 2020-09-23
Answer
The animal species the immunogen sequence for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is Human.
Boster Scientific Support
Answered: 2020-09-23
Question
What is the Expression host of the immunogen for the detection antibody of EK1307?
Verified customer
Asked: 2020-09-23
Answer
The Expression host of the immunogen for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is goat.
Boster Scientific Support
Answered: 2020-09-23
Question
Is the Immunogen for the detection antibody of EK1307 native or recombinant?
Verified customer
Asked: 2020-09-23
Answer
The Immunogen for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is recombinant.
Boster Scientific Support
Answered: 2020-09-23
Question
Is the Immunogen for the detection antibody of EK1307 full-length or partial?
Verified customer
Asked: 2020-09-23
Answer
The Immunogen for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is partial.
Boster Scientific Support
Answered: 2020-09-23
Question
What animal species for the standard immunogen sequence of EK1307 comes from?
Verified customer
Asked: 2020-09-23
Answer
The animal species for the standard immunogen sequence of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is Human?
Boster Scientific Support
Answered: 2020-09-23
Question
What is the Expression host for the standard immunogen sequence of EK1307?
Verified customer
Asked: 2020-09-23
Answer
The Expression host for the standard immunogen sequence of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is NSO.
Boster Scientific Support
Answered: 2020-09-23
Question
Is the Immunogen standard of EK1307 native or recombinant?
Verified customer
Asked: 2020-09-23
Answer
The Immunogen standard of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is recombinant.
Boster Scientific Support
Answered: 2020-09-23
Question
Is the Immunogen standard of EK1307 full-length or partial?
Verified customer
Asked: 2020-09-23
Answer
The Immunogen standard of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is partial.
Boster Scientific Support
Answered: 2020-09-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-09-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-06
Question
Q: can I use citrate plasma as samples in Human TGF-beta RII Picokine® ELISA Kit (Catalog # EK1307)?
K. Wilson
Verified customer
Asked: 2020-06-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TGF-beta RII causing degradation of its protein structure. TGF-beta RII may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TGF-beta RII ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement TGF-beta RII using the EK1307 Human TGF-beta RII Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-09-12
Question
Q: which procedure should I follow in order to thaw whole blood sample for TGF-beta RII ELISA after freezing?
C. Harris
Verified customer
Asked: 2018-07-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGF-beta RII for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-07-11
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-01-24
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-24
