Anti-TEM5 ADGRA2 Antibody

Product Name

Anti-TEM5 ADGRA2 Antibody

View all TEM5/GPR124 Antibodies

SKU/Catalog Number

A09540

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TEM5 ADGRA2 Antibody (Catalog # A09540). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Rat.

Storage & Handling

TEM5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TEM5 ADGRA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09540)

Host

Rabbit

Contents

TEM5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TEM5 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human TEM5. The immunogen is located within amino acids 1270 - 1320 of TEM5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.

Reactive Species

A09540 is reactive to GPR124 in Human, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

142647 MW

Background of TEM5/GPR124

Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A09540 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For GPR124 (Source: Uniprot.org, NCBI)

Alternative Names

TEM5, KIAA1531, TEM5, G-protein coupled receptor 124, Tumor endothelial marker 5 ADGRA2 GPR124, TEM5 adhesion G protein-coupled receptor A2 adhesion G protein-coupled receptor A2|G-protein coupled receptor 124|tumor endothelial marker 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis