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Product Info Summary
| SKU: | A01309-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-KDM6B/JMJD3 Picoband® Antibody
View all Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibodies
SKU/Catalog Number
A01309-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KDM6B/JMJD3 Picoband® Antibody catalog # A01309-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KDM6B/JMJD3 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01309-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01309-1 is reactive to KDM6B in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
177 kDa
Calculated molecular weight
18012 MW
Background of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3
Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01309-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human K562 whole cell, human A375 whole cell, human HEK293 whole cell, human A431 whole cell
FCM: K562 cell, HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody (A01309-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM6B antigen affinity purified polyclonal antibody (Catalog # A01309-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM6B at approximately 177KD. The expected band size for KDM6B is at 177KD.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-KDM6B antibody (A01309-1).
Overlay histogram showing K562 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM6B Antibody (A01309-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-KDM6B antibody (A01309-1).
Overlay histogram showing HEPA1-6 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM6B Antibody (A01309-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For KDM6B (Source: Uniprot.org, NCBI)
Gene Name
KDM6B
Full Name
Lysine-specific demethylase 6B
Weight
18012 MW
Superfamily
UTX family
Alternative Names
Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B; KDM6B; JMJD3; KIAA0346 KDM6B JMJD3, NEDCFSA lysine demethylase 6B lysine-specific demethylase 6B|[histone H3]-trimethyl-L-lysine(27) demethylase 6B|jmjC domain-containing protein 3|jumonji domain containing 3, histone lysine demethylase|jumonji domain-containing protein 3|lysine (K)-specific demethylase 6B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM6B, check out the KDM6B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM6B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KDM6B/JMJD3 Picoband® Antibody (A01309-1)
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