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[74] => cj_related_products
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[78] => contents
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[86] => competitor_equivalent_skus
[87] => immunogen
[88] => purification
[89] => storage
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Key=store_id Key=entity_id, value=3475 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=PB9824 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2015-12-31 20:13:49 Key=updated_at, value=2024-09-26 00:46:58 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-TAP2 Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1369 Key=yoast_content_score, value=90 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-TAP2 Antibody Picoband® | Bosterbio Key=meta_description, value=Boster Bio Anti-TAP2 Antibody Picoband® catalog # PB9824. Tested in WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/p/b/pb9824-1.jpg Key=small_image, value=/p/b/pb9824-1.jpg Key=thumbnail, value=/p/b/pb9824-1.jpg Key=url_key, value=anti-tap2-picoband-trade-antibody-pb9824-boster Key=url_path, value=anti-tap2-picoband-trade-antibody-pb9824-boster Key=options_container, value=Product Info Column Key=image_label, value=Anti-TAP2 Picoband antibody, PB9824, Western blotting All lanes: Anti TAP2 (PB9824) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD Key=small_image_label, value=Anti-TAP2 Picoband antibody, PB9824, Western blotting All lanes: Anti TAP2 (PB9824) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD Key=thumbnail_label, value=Anti-TAP2 Picoband antibody, PB9824, Western blotting All lanes: Anti TAP2 (PB9824) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Antigen peptide transporter 2 Key=gene_full_name, value=Antigen peptide transporter 2 Key=uniprot_id, value=Q03519 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=WB Key=reactivity, value=Human Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=predicted_reactivity Key=source_company, value=Boster PB9824 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=75664 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Chaperones, ER Proteins, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers, Tags & Cell Markers Key=clonality, value=Polyclonal Key=swatch_image, value=/antibody/ek1118-2-ELISA-human-a2m-alpha2-macroglobulin-picokine-elisa-kit.jpg Key=rating_value, value=95 Key=review_count, value=3 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_focus_keyword, value=CD68 antibody Key=yoast_facebook_image, value=/p/b/pb9800-cd19-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/p/b/pb9800-cd19-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Antigen Processing And Presentation:PA1490^Cell Recognition:PA1850|A00323^Tumor Escape:PB9553|PB9994|A00109 Key=description, value=Boster Bio Anti-TAP2 Antibody Picoband® catalog # PB9824. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-TAP2 Antibody Picoband® catalog # PB9824. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-TAP2 Antibody Picoband® Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Key=background, value=Transporter, ATP-binding cassette, major histocompatibility complex 2 (TAP2) is a gene in humans that encodes the protein Antigen peptide transporter 2. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The gene is assigned to human chromosome 6p21.3. It is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. And this protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Key=gene_name, value=TAP2 Key=synonyms, value=Antigen peptide transporter 2;APT2;ATP-binding cassette sub-family B member 3;Peptide supply factor 2;Peptide transporter PSF2;PSF-2;Peptide transporter TAP2;Peptide transporter involved in antigen processing 2;Really interesting new gene 11 protein;TAP2;ABCB3, PSF2, RING11, Y1; Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human
Key=subcellular_localization, value= Endoplasmic reticulum membrane; Multi-pass membrane protein. The transmembrane segments seem to form a pore in the membrane. Key=tissue_specificity, value= Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung. . Key=application_notes, value=Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 25612|sc 11473|sc 11474 Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human TAP2, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids. Key=purification, value=Immunogen affinity purified. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=87 kDa Key=custom_attribute_2, value=WB: HELA Whole Cell Key=custom_attribute_3, value=PB0869 Key=protein_function, value= Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-TAP2 Antibody Picoband®
TAP2 antibody
Boster Bio Anti-TAP2 Antibody Picoband® catalog # PB9824. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Boster Bio Anti-TAP2 Antibody Picoband® catalog # PB9824. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9824)
A synthetic peptide corresponding to a sequence at the C-terminus of human TAP2, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9824 is reactive to TAP2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
75664 MW
Background of TAP2
Transporter, ATP-binding cassette, major histocompatibility complex 2 (TAP2) is a gene in humans that encodes the protein Antigen peptide transporter 2. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The gene is assigned to human chromosome 6p21.3. It is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. And this protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TAP2 using anti-TAP2 antibody (PB9824).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP2 antigen affinity purified polyclonal antibody (Catalog # PB9824) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAP2 at approximately 87 kDa. The expected band size for TAP2 is at 87 kDa.
Gene/Protein Information For TAP2 (Source: Uniprot.org, NCBI)
Gene Name
TAP2
Full Name
Antigen peptide transporter 2
Weight
75664 MW
Superfamily
ABC transporter superfamily
Alternative Names
Antigen peptide transporter 2;APT2;ATP-binding cassette sub-family B member 3;Peptide supply factor 2;Peptide transporter PSF2;PSF-2;Peptide transporter TAP2;Peptide transporter involved in antigen processing 2;Really interesting new gene 11 protein;TAP2;ABCB3, PSF2, RING11, Y1; TAP2 ABC18, ABCB3, APT2, D6S217E, PSF-2, PSF2, RING11 transporter 2, ATP binding cassette subfamily B member peptide transporter 2|ABC transporter, MHC 2|ATP-binding cassette, sub-family B (MDR/TAP), member 3|peptide supply factor 2|peptide transporter PSF2|peptide transporter involved in processing 2|really interesting new gene 11 protein|transporter 2, ABC (ATP binding cassette)|transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on TAP2, check out the TAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
We are currently using anti-TAP2 antibody PB9824 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-TAP2 antibody (PB9824) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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