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Product Info Summary
| SKU: | A01123 |
|---|---|
| Size: | 100µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Antigen peptide transporter 1 TAP1 Antibody
SKU/Catalog Number
A01123
Size
100µl
Form
Liquid. Whole rabbit serum.
Description
Boster Bio Anti-Antigen peptide transporter 1 TAP1 Antibody catalog # A01123. Tested in IP, IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Antigen peptide transporter 1 TAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01123)
Host
Rabbit
Contents
Liquid. Whole rabbit serum.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide corresponding to a portion of human TAP1 .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01123 is reactive to TAP1 in Human, Rat
Reconstitution
Calculated molecular weight
31083 MW
Background of TAP1
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Scanlan M.J., Cancer Immun. 1:4-4(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Baas A.F., EMBO J. 22:3062-3072(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01123 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunoprecipitation (1:100)
Western Blot (ECL, 1:1,000-1:4000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Figure 1. IHC analysis of TAP1 using anti-TAP1 antibody (A01123).
TAP1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAP1 Antibody (A01123) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of TAP1 using anti-TAP1 antibody (A01123).
TAP1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAP1 Antibody (A01123) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TAP1 using anti-TAP1 antibody (A01123).
TAP1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAP1 Antibody (A01123) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Western blot analysis of TAP1 using anti-TAP1 antibody (A01123).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP1 antigen affinity purified polyclonal antibody (Catalog # A01123) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for TAP1.
Protein Target Info & Infographic
Gene/Protein Information For TAP1 (Source: Uniprot.org, NCBI)
Gene Name
TAP1
Full Name
Antigen peptide transporter 1
Weight
31083 MW
Superfamily
ABC transporter superfamily
Alternative Names
Transcription factor PU.1;31 kDa-transforming protein;SPI1; TAP1 ABC17, ABCB2, APT1, D6S114E, PSF-1, PSF1, RING4*0102N, TAP1N, TAP1 transporter 1, ATP binding cassette subfamily B member peptide transporter 1|ABC transporter, MHC 1|ATP-binding cassette sub-family B member 2|ATP-binding cassette, sub-family B (MDR/TAP), member 2|peptide supply factor 1|peptide transporter PSF1|peptide transporter TAP1|peptide transporter involved in processing 1|really interesting new gene 4 protein|transporter 1 ATP-binding cassette sub-family B|transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|transporter associated with processing|transporter, ATP-binding cassette, major histocompatibility complex, 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAP1, check out the TAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Antigen peptide transporter 1 TAP1 Antibody (A01123)
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Customer Q&As
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6 Customer Q&As for Anti-Antigen peptide transporter 1 TAP1 Antibody
Question
Does A01123 anti-TAP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
As indicated on the product datasheet, A01123 anti-TAP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-25
Question
See attached the WB image, lot number and protocol we used for blood using anti-TAP1 antibody A01123. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
Do you have a BSA free version of anti-TAP1 antibody A01123 available?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TAP1 antibody A01123 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-05
Question
Does anti-TAP1 antibody A01123 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
According to the expression profile of blood, TAP1 is highly expressed in blood. So, it is likely that anti-TAP1 antibody A01123 will work for WB with blood.
Boster Scientific Support
Answered: 2019-10-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-TAP1 antibody A01123. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-26
Question
Is a blocking peptide available for product anti-TAP1 antibody (A01123)?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
We do provide the blocking peptide for product anti-TAP1 antibody (A01123). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-24
