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Product Info Summary
| SKU: | A02314 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Synaptotagmin 1 (Ab-309) SYT1 Antibody
View all Synaptotagmin 1 Antibodies
SKU/Catalog Number
A02314
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Synaptotagmin 1 (Ab-309) SYT1 Antibody (Catalog # A02314). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptotagmin 1 (Ab-309) SYT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02314)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.307~311 (G-L-S-D-P) derived from Human Synaptotagmin 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02314 is reactive to SYT1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
95338 MW
Background of Synaptotagmin 1
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse
Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7.
Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31.
Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02314 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 60-65kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1 (Ab-309) Antibody #A02314.
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Figure 2. Western blot analysis of SYT1 using anti-SYT1 antibody (A02314).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYT1 antigen affinity purified polyclonal antibody (Catalog # A02314) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SYT1.
Protein Target Info & Infographic
Gene/Protein Information For SYT1 (Source: Uniprot.org, NCBI)
Gene Name
SYT1
Full Name
Synaptotagmin-1
Weight
95338 MW
Superfamily
synaptotagmin family
Alternative Names
Interleukin enhancer-binding factor 3;Double-stranded RNA-binding protein 76;DRBP76;M-phase phosphoprotein 4;MPP4;Nuclear factor associated with dsRNA;NFAR;Nuclear factor of activated T-cells 90 kDa;NF-AT-90;Translational control protein 80;TCP80;ILF3;DRBF, MPHOSPH4, NF90; SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT1, check out the SYT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptotagmin 1 (Ab-309) SYT1 Antibody (A02314)
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No publications found for A02314
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Customer Q&As
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16 Customer Q&As for Anti-Synaptotagmin 1 (Ab-309) SYT1 Antibody
Question
We ordered your anti-Synaptotagmin 1 (Ab-309) antibody for WB on hippocampus a few months ago. I am using mouse, and We want to use the antibody for IF next. I am interested in examining hippocampus as well as amygdala brain in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
I viewed the website and datasheets of our anti-Synaptotagmin 1 (Ab-309) antibody and it seems that A02314 has been validated on mouse in both WB and IF. Thus A02314 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-19
Question
I am interested in using your anti-Synaptotagmin 1 (Ab-309) antibody for synchronous neurotransmitter secretion studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
R. Brown
Verified customer
Asked: 2020-03-09
Answer
Thank you for your inquiry. This A02314 anti-Synaptotagmin 1 (Ab-309) antibody is validated on hela cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-09
Question
See below the WB image, lot number and protocol we used for pns using anti-Synaptotagmin 1 (Ab-309) antibody A02314. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-17
Question
Does A02314 anti-Synaptotagmin 1 (Ab-309) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
As indicated on the product datasheet, A02314 anti-Synaptotagmin 1 (Ab-309) antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-03
Question
I was wanting to use your anti-Synaptotagmin 1 (Ab-309) antibody for IF for human pns on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pns identification?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
You can see on the product datasheet, A02314 anti-Synaptotagmin 1 (Ab-309) antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pns in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
I see that the anti-Synaptotagmin 1 (Ab-309) antibody A02314 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-10
Question
My colleagues were content with the WB result of your anti-Synaptotagmin 1 (Ab-309) antibody. However we have seen positive staining in pns cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
According to literature, pns does express SYT1. Generally SYT1 expresses in cytoplasmic vesicle, secretory vesicle. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-16
Question
We have seen staining in human pns. What should we do? Is anti-Synaptotagmin 1 (Ab-309) antibody supposed to stain pns positively?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
According to literature pns does express SYT1. According to Uniprot.org, SYT1 is expressed in middle temporal gyrus, amygdala brain, hippocampus, pns, among other tissues. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-07
Question
Is there a BSA free version of anti-Synaptotagmin 1 (Ab-309) antibody A02314 available?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Synaptotagmin 1 (Ab-309) antibody A02314 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-16
Question
Would anti-Synaptotagmin 1 (Ab-309) antibody A02314 work for IF with pns?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
According to the expression profile of pns, SYT1 is highly expressed in pns. So, it is likely that anti-Synaptotagmin 1 (Ab-309) antibody A02314 will work for IF with pns.
Boster Scientific Support
Answered: 2019-08-09
Question
I have a question about product A02314, anti-Synaptotagmin 1 (Ab-309) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02314 anti-Synaptotagmin 1 (Ab-309) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pns using anti-Synaptotagmin 1 (Ab-309) antibody A02314. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-25
Question
We want to test anti-Synaptotagmin 1 (Ab-309) antibody A02314 on human pns for research purposes, then I may be interested in using anti-Synaptotagmin 1 (Ab-309) antibody A02314 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The products we sell, including anti-Synaptotagmin 1 (Ab-309) antibody A02314, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-21
Question
We are currently using anti-Synaptotagmin 1 (Ab-309) antibody A02314 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The anti-Synaptotagmin 1 (Ab-309) antibody (A02314) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-08
Question
Is this A02314 anti-Synaptotagmin 1 (Ab-309) antibody reactive to the isotypes of SYT1?
B. Anderson
Verified customer
Asked: 2017-04-03
Answer
The immunogen of A02314 anti-Synaptotagmin 1 (Ab-309) antibody is Peptide sequence around aa.307~311 (G-L-S-D-P) derived from Human Synaptotagmin 1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-03
Question
Is a blocking peptide available for product anti-Synaptotagmin 1 (Ab-309) antibody (A02314)?
K. Williams
Verified customer
Asked: 2013-10-23
Answer
We do provide the blocking peptide for product anti-Synaptotagmin 1 (Ab-309) antibody (A02314). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-23
