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Product Info Summary
| SKU: | A02314-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Synaptotagmin 1 DyLight® 488 conjugated SYT1 Antibody
View all Synaptotagmin 1 Antibodies
SKU/Catalog Number
A02314-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Synaptotagmin 1 DyLight® 488 conjugated SYT1 Antibody catalog # A02314-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Synaptotagmin 1 DyLight® 488 conjugated SYT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02314-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptotagmin 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02314-Dyl488 is reactive to SYT1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
30392 MW
Background of Synaptotagmin 1
Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02314-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For SYT1 (Source: Uniprot.org, NCBI)
Gene Name
SYT1
Full Name
Synaptotagmin-1
Weight
30392 MW
Superfamily
synaptotagmin family
Alternative Names
Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT1; SVP65; SYT SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT1, check out the SYT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Synaptotagmin 1 DyLight® 488 conjugated SYT1 Antibody (A02314-Dyl488)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Synaptotagmin 1 DyLight® 488 conjugated SYT1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus using anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
Does anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 work for Flow Cytometry with hippocampus?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to the expression profile of hippocampus, SYT1 is highly expressed in hippocampus. So, it is likely that anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 will work for Flow Cytometry with hippocampus.
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody (A02314-Dyl488) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
Is there a BSA free version of anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 available?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-28
Question
Would A02314-Dyl488 anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Lewis
Verified customer
Asked: 2020-01-27
Answer
As indicated on the product datasheet, A02314-Dyl488 anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-27
Question
We have been able to see staining in human middle temporal gyrus. Do you have any suggestions? Is anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody supposed to stain middle temporal gyrus positively?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
According to literature middle temporal gyrus does express SYT1. According to Uniprot.org, SYT1 is expressed in middle temporal gyrus, amygdala brain, hippocampus, pns, among other tissues. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-21
Question
I have a question about product A02314-Dyl488, anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02314-Dyl488 anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-25
Question
Is a blocking peptide available for product anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody (A02314-Dyl488)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do provide the blocking peptide for product anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody (A02314-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use your anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody for Flow Cytometry for human hippocampus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus identification?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
As indicated on the product datasheet, A02314-Dyl488 anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-01
Question
Is this A02314-Dyl488 anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody reactive to the isotypes of SYT1?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
The immunogen of A02314-Dyl488 anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody is A synthetic peptide corresponding to a sequence of human Synaptotagmin 1 (RRPIAQWHTLQVEEEVDAMLAVKK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-08
Question
We want to test anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 on human hippocampus for research purposes, then I may be interested in using anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Jones
Verified customer
Asked: 2016-06-24
Answer
The products we sell, including anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-06-24
Question
Please see the WB image, lot number and protocol we used for hippocampus using anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488. Please let me know if you require anything else.
E. Roberts
Verified customer
Asked: 2014-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-22
Question
My colleagues were well pleased with the WB result of your anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody. However we have been able to see positive staining in hippocampus cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYT1 supposed to be expressed?
M. Johnson
Verified customer
Asked: 2013-10-25
Answer
Based on literature, hippocampus does express SYT1. Generally SYT1 expresses in cytoplasmic vesicle, secretory vesicle. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-10-25
Question
I see that the anti-Human Synaptotagmin 1 DyLight® 488 conjugated antibody A02314-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
D. Parker
Verified customer
Asked: 2013-09-10
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-10
