Product Info Summary
SKU: | A02314-Dyl550 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody
View all Synaptotagmin 1 Antibodies
SKU/Catalog Number
A02314-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody catalog # A02314-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02314-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptotagmin 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02314-Dyl550 is reactive to SYT1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Background of Synaptotagmin 1
Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02314-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
![boster box boster box](https://www.bosterbio.com/media/catalog/product/b/o/boster-box.png)
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For SYT1 (Source: Uniprot.org, NCBI)
Gene Name
SYT1
Full Name
Synaptotagmin-1
Weight
Superfamily
synaptotagmin family
Alternative Names
DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT1, check out the SYT1 Infographic
![SYT1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody (A02314-Dyl550)
Hello CJ!
No publications found for A02314-Dyl550
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
14 Customer Q&As for Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody
Question
Do you have a BSA free version of anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 available?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-05
Question
Would A02314-Dyl550 anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
It shows on the product datasheet, A02314-Dyl550 anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-03
Question
See below the WB image, lot number and protocol we used for amygdala brain using anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-02
Question
I see that the anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-27
Question
Is this A02314-Dyl550 anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody reactive to the isotypes of SYT1?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The immunogen of A02314-Dyl550 anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody is A synthetic peptide corresponding to a sequence of human Synaptotagmin 1 (RRPIAQWHTLQVEEEVDAMLAVKK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-22
Question
Will anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 work for Flow Cytometry with amygdala brain?
H. Krishna
Verified customer
Asked: 2019-11-13
Answer
According to the expression profile of amygdala brain, SYT1 is highly expressed in amygdala brain. So, it is likely that anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 will work for Flow Cytometry with amygdala brain.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody for Flow Cytometry for human amygdala brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amygdala brain identification?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
As indicated on the product datasheet, A02314-Dyl550 anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-21
Question
We have been able to see staining in human middle temporal gyrus. What should we do? Is anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody supposed to stain middle temporal gyrus positively?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
According to literature middle temporal gyrus does express SYT1. According to Uniprot.org, SYT1 is expressed in middle temporal gyrus, amygdala brain, hippocampus, pns, among other tissues. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-31
Question
We were happy with the WB result of your anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody. However we have been able to see positive staining in hippocampus cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
From what I have seen in literature, hippocampus does express SYT1. Generally SYT1 expresses in cytoplasmic vesicle, secretory vesicle. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-27
Question
My question regarding product A02314-Dyl550, anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02314-Dyl550 anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala brain using anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-02
Question
I am interested in to test anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 on human amygdala brain for research purposes, then I may be interested in using anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
The products we sell, including anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-24
Question
Is a blocking peptide available for product anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody (A02314-Dyl550)?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
We do provide the blocking peptide for product anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody (A02314-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-26
Question
We are currently using anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody A02314-Dyl550 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
C. Zhang
Verified customer
Asked: 2017-05-24
Answer
The anti-Human Synaptotagmin 1 DyLight® 550 conjugated antibody (A02314-Dyl550) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-24