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Product Info Summary
| SKU: | PB10017 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SYBL1/VAMP7 Antibody Picoband®
SKU/Catalog Number
PB10017
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SYBL1/VAMP7 Antibody Picoband® catalog # PB10017. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SYBL1/VAMP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10017)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SYBL1 recombinant protein (Position: N24-T190). Human SYBL1 shares 97.6% and 95.2% amino acid (aa) sequence identity with mouse and rat SYBL1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10017 is reactive to VAMP7 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
24935 MW
Background of VAMP-7
Vesicle-associated membrane protein 7 (VAMP-7), also known as SYBL1, is a protein that in humans is encoded by the VAMP7 gene. This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide- sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10017 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat liver tissue, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SYBL1 using anti-SYBL1 antibody (PB10017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYBL1 antigen affinity purified polyclonal antibody (Catalog # PB10017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYBL1 at approximately 25 kDa. The expected band size for SYBL1 is at 25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For VAMP7 (Source: Uniprot.org, NCBI)
Gene Name
VAMP7
Full Name
Vesicle-associated membrane protein 7
Weight
24935 MW
Superfamily
synaptobrevin family
Alternative Names
Vesicle-associated membrane protein 7;VAMP-7;Synaptobrevin-like protein 1;Tetanus-insensitive VAMP;Ti-VAMP;VAMP7;SYBL1; VAMP7 SYBL1, TI-VAMP, TIVAMP, VAMP-7 vesicle associated membrane protein 7 vesicle-associated membrane protein 7|synaptobrevin-like 1|synaptobrevin-like protein 1|tetanus neurotoxin-insensitive VAMP|tetanus-insensitive VAMP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VAMP7, check out the VAMP7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VAMP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SYBL1/VAMP7 Antibody Picoband® (PB10017)
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Customer Q&As
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6 Customer Q&As for Anti-SYBL1/VAMP7 Antibody Picoband®
Question
We are currently using anti-SYBL1/VAMP7 antibody PB10017 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-SYBL1/VAMP7 antibody (PB10017) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
Can you help my question with product PB10017, anti-SYBL1/VAMP7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10017 anti-SYBL1/VAMP7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
I was wanting to use your anti-SYBL1/VAMP7 antibody for WB for human hippocampus uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus uterus identification?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
It shows on the product datasheet, PB10017 anti-SYBL1/VAMP7 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
Would anti-SYBL1/VAMP7 antibody PB10017 work for WB with hippocampus uterus?
K. Jackson
Verified customer
Asked: 2018-08-23
Answer
According to the expression profile of hippocampus uterus, VAMP7 is highly expressed in hippocampus uterus. So, it is likely that anti-SYBL1/VAMP7 antibody PB10017 will work for WB with hippocampus uterus.
Boster Scientific Support
Answered: 2018-08-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus uterus using anti-SYBL1/VAMP7 antibody PB10017. Let me know if you need anything else.
Z. Anderson
Verified customer
Asked: 2018-05-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-23
Question
Is this PB10017 anti-SYBL1/VAMP7 antibody reactive to the isotypes of VAMP7?
G. Thomas
Verified customer
Asked: 2015-05-25
Answer
The immunogen of PB10017 anti-SYBL1/VAMP7 antibody is E. coli-derived human SYBL1 recombinant protein (Position: N24-T190). Human SYBL1 shares 97.6% and 95.2% amino acid (aa) sequence identity with mouse and rat SYBL1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-25
