Human PDGF-AB PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human PDGF-AB PicoKine® Quick ELISA Kit

See all PDGF-AB products

SKU/Catalog Number

FEK0484

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PDGF-AB PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PDGF-AB PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0484)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190)

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0484 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0484 is reactive to PDGF-AB in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0484 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of PDGF-AB

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.

Kit Components

Catalog number	Description	Quantity
FEK0484-CAP	96-well plate precoated with anti-Human PDGF-AB antibody	1
EK0484-ST	lyophilized recombinant Human PDGF-AB standard	10 ng/tube
FEK0484-DA	Biotinylated anti-Human PDGF-AB detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.023	0.126	0.252	0.423	0.922	1.358	1.856	2.095

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PDGF-AB PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PDGF-AB in ELISA kits from four different production batches/lots.

Gene/Protein Information For PDGF-AB (Source: Uniprot.Org, NCBI)

Alternative Names

Platelet-derived growth factor subunit B;PDGF subunit B;PDGF-2;Platelet-derived growth factor B chain;Platelet-derived growth factor beta polypeptide;Proto-oncogene c-Sis;Becaplermin;PDGFB;PDGF2, SIS;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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