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Product Info Summary
| SKU: | A02849 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Bovine, Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-SmcX KDM5C Monoclonal Antibody
SKU/Catalog Number
A02849
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SmcX KDM5C Monoclonal Antibody catalog # A02849. Tested in IF, WB applications. This antibody reacts with Bovine, Dog, Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SmcX KDM5C Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02849)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
4E7E1
Isotype
IgG
Immunogen
Purified recombinant human SmcX (C-terminus) protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02849 is reactive to KDM5C in Bovine, Dog, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
175720 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02849 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IF 1:100-1:500
Validation Images & Assay Conditions
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Western Blot (WB) analysis using SmcX Monoclonal antibody against HeLa, 293 cell lysate.
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Immunofluorescence (IF) analysis of HeLa cells using SmcX Monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For KDM5C (Source: Uniprot.org, NCBI)
Gene Name
KDM5C
Full Name
Lysine-specific demethylase 5C
Weight
175720 MW
Superfamily
JARID1 histone demethylase family
Alternative Names
Lysine-specific demethylase 5C;1.14.11.-;Histone demethylase JARID1C;Jumonji/ARID domain-containing protein 1C;Protein SmcX;Protein Xe169;KDM5C;DXS1272E, JARID1C, SMCX, XE169; KDM5C DXS1272E, JARID1C, MRX13, MRXJ, MRXSCJ, MRXSJ, SMCX, XE169 lysine demethylase 5C lysine-specific demethylase 5C|JmjC domain-containing protein SMCX|Jumonji, AT rich interactive domain 1C (RBP2-like)|Jumonji/ARID domain-containing protein 1C|Smcx homolog, X chromosome|Smcy homolog, X-linked|[histone H3]-trimethyl-L-lysine(4) demethylase 5C|histone demethylase JARID1C|lysine (K)-specific demethylase 5C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM5C, check out the KDM5C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM5C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SmcX KDM5C Monoclonal Antibody (A02849)
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No publications found for A02849
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SmcX KDM5C Monoclonal Antibody
Question
Can you help my question with product A02849, anti-SmcX Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02849 anti-SmcX Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-08
Question
We are currently using anti-SmcX Monoclonal antibody A02849 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-SmcX Monoclonal antibody (A02849) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left ovary using anti-SmcX Monoclonal antibody A02849. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
See attached the WB image, lot number and protocol we used for left ovary using anti-SmcX Monoclonal antibody A02849. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-18
Question
Is this A02849 anti-SmcX Monoclonal antibody reactive to the isotypes of KDM5C?
M. Rodriguez
Verified customer
Asked: 2018-02-07
Answer
The immunogen of A02849 anti-SmcX Monoclonal antibody is Purified recombinant human SmcX (C-terminal) protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-07
Question
Do you have a BSA free version of anti-SmcX Monoclonal antibody A02849 available?
J. Miller
Verified customer
Asked: 2015-10-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SmcX Monoclonal antibody A02849 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-10-16
Question
I was wanting to use your anti-SmcX Monoclonal antibody for IF for bovine left ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for bovine left ovary identification?
A. Mangal
Verified customer
Asked: 2013-07-15
Answer
As indicated on the product datasheet, A02849 anti-SmcX Monoclonal antibody has been tested for IF, IHC, WB on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine left ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-15
