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Product Info Summary
| SKU: | M04267 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SULF1 Antibody (C-Term)
View all Sulfatase 1 Antibodies
SKU/Catalog Number
M04267
Size
50 µl
Description
Boster Bio Anti-SULF1 Antibody (C-Term) (Catalog # M04267). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SULF1 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04267)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SULF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 704-738 amino acids from human SULF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04267 is reactive to SULF1 in Human
Reconstitution
Calculated molecular weight
101027 Da
Background of Sulfatase 1
Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04267 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Anti-SULF1 Antibody (C-Term) at 1:2000 dilution + human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 101 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-251 MG cells stained with M04267(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M04267, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For SULF1 (Source: Uniprot.org, NCBI)
Gene Name
SULF1
Full Name
Extracellular sulfatase Sulf-1
Weight
101027 Da
Superfamily
sulfatase family
Alternative Names
Extracellular sulfatase Sulf-1, hSulf-1, 3.1.6.-, SULF1, KIAA1077 SULF1 SULF-1 sulfatase 1 extracellular sulfatase Sulf-1|sulfatase FP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SULF1, check out the SULF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SULF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SULF1 Antibody (C-Term) (M04267)
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