Anti-HLF Antibody Picoband®

HLF antibody

Boster Bio Anti-HLF Antibody Picoband® catalog # A01284-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01284-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-HLF Antibody Picoband®

View all HLF Antibodies

SKU/Catalog Number

A01284-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLF Antibody Picoband® catalog # A01284-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01284-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HLF, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01284-1 is reactive to HLF in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

33 kDa

Calculated molecular weight

33.199kDa

Background of HLF

Hepatic leukemia factor is a protein that in humans is encoded by the HLF gene. This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01284-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Positive Control

WB: human placenta tissue, rat brain tissue, rat liver tissue, rat kidney tissue, rat lung whole cell, mouse brain tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue

Validation Images & Assay Conditions

Gene/Protein Information For HLF (Source: Uniprot.org, NCBI)

Gene Name

HLF

Full Name

Hepatic leukemia factor

Weight

33.199kDa

Superfamily

bZIP family

Alternative Names

Hepatic leukemia factor; HLF HLF HLF transcription factor, PAR bZIP family member hepatic leukemia factor|HLF, PAR bZIP transcription factor|PAR bZIP transcription factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HLF, check out the HLF Infographic

HLF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HLF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-HLF Antibody Picoband®

Question

We are currently using anti-HLF antibody A01284-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

The anti-HLF antibody (A01284-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-13

Question

Is there a BSA free version of anti-HLF antibody A01284-1 available?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLF antibody A01284-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-18

Question

Is a blocking peptide available for product anti-HLF antibody (A01284-1)?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

We do provide the blocking peptide for product anti-HLF antibody (A01284-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-12-19

Question

Is this A01284-1 anti-HLF antibody reactive to the isotypes of HLF?

Verified Customer

Verified customer

Asked: 2017-09-26

Answer

The immunogen of A01284-1 anti-HLF antibody is A synthetic peptide corresponding to a sequence of human HLF (DDLKDDKYWARRRKNNMAAKRSRDARRLKE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-09-26

Question

Will A01284-1 anti-HLF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-19

Answer

You can see on the product datasheet, A01284-1 anti-HLF antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-19

Question

Does anti-HLF antibody A01284-1 work on primate WB with hepatoma?

L. Moore

Verified customer

Asked: 2016-03-23

Answer

Our lab technicians have not tested anti-HLF antibody A01284-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-HLF antibody A01284-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate hepatoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-03-23

Question

I was wanting to use your anti-HLF antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat frontal cortex identification?

M. Wu

Verified customer

Asked: 2013-10-16

Answer

You can see on the product datasheet, A01284-1 anti-HLF antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-10-16

Order DetailsPrice
A01284-1

100μg

$370
A01284-1-10ug

10μg sample (liquid)

$99
A01284-1-Biotin

100 μg Biotin conjugated

$570
A01284-1-Cy3

100 μg Cy3 conjugated

$570
A01284-1-Dylight488

100 μg Dylight488 conjugated

$570
A01284-1-Dylight550

100 μg Dylight550 conjugated

$570
A01284-1-Dylight594

100 μg Dylight594 conjugated

$570
A01284-1-FITC

100 μg FITC conjugated

$570
A01284-1-HRP

100 μg HRP conjugated

$570
A01284-1-APC

100 μg APC conjugated

$670
A01284-1-PE

100 μg PE conjugated

$670
A01284-1-iFluor647

100 μg iFluor647 conjugated

$670
A01284-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A01284-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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