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Product Info Summary
| SKU: | M04729 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-STMN2 Antibody (Center)
View all Stathmin-2/STMN2 Antibodies
SKU/Catalog Number
M04729
Size
80 µl
Description
Boster Bio Anti-STMN2 Antibody (Center) (Catalog # M04729). Tested in WB, IHC, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-STMN2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04729)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This STMN2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 82-116 amino acids from the Central region of human STMN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04729 is reactive to STMN2 in Human, Mouse
Reconstitution
Calculated molecular weight
20828 Da
Background of Stathmin-2/STMN2
Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04729 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized SH-SY5Y (Human metastatic neuroblastoma cell line) cells labeling STMN2 with M04729 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasm on SH-SY5Y cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).
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All lanes : Anti-STMN2 Antibody (Center) at 1:1000 dilution
Lane 1: human brain lysates
Lane 2: mouse brain lysates
Lane 3: SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M04729 staining STMN2 in Human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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M04729 staining STMN2 in Human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing SH-SY5Y cells stained with M04729 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For STMN2 (Source: Uniprot.org, NCBI)
Gene Name
STMN2
Full Name
Stathmin-2
Weight
20828 Da
Superfamily
stathmin family
Alternative Names
Stathmin-2, Superior cervical ganglion-10 protein, Protein SCG10, STMN2, SCG10, SCGN10 STMN2 SCG10, SCGN10 stathmin 2 stathmin-2|neuron-specific growth-associated protein|neuronal growth-associated protein (silencer element)|stathmin-like 2|superior cervical ganglia, neural specific 10|superior cervical ganglion-10 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STMN2, check out the STMN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STMN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STMN2 Antibody (Center) (M04729)
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