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Product Info Summary
| SKU: | A04219 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-14-3-3 eta YWHAH Antibody
View all 14-3-3 eta Antibodies
SKU/Catalog Number
A04219
Size
100ul
Form
Liquid
Description
Boster Bio Anti-14-3-3 eta YWHAH Antibody (Catalog# A04219). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 eta YWHAH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04219)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human 14-3-3 eta. Immunogen sequence location: 51-100
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04219 is reactive to YWHAH in Human, Mouse, Rat
Applications
A04219 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
28219 MW
Background of 14-3-3 eta
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using 14-3-3 eta Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Protein Target Info & Infographic
Gene/Protein Information For YWHAH (Source: Uniprot.org, NCBI)
Gene Name
YWHAH
Full Name
14-3-3 protein eta
Weight
28219 MW
Superfamily
14-3-3 family
Alternative Names
1433 eta; 14-3-3 eta; 14-3-3 protein eta; Protein AS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide; YWHA1; YWHA114-3-3 eta; YWHAH YWHAH YWHA1 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta 14-3-3 protein eta|14-3-3 eta|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YWHAH, check out the YWHAH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YWHAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-14-3-3 eta YWHAH Antibody (A04219)
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No publications found for A04219
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