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Product Info Summary
| SKU: | M04219 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Canine, Equine, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-1433 Eta (eta) Monoclonal Antibody
View all 14-3-3 eta Antibodies
SKU/Catalog Number
M04219
Size
100ul
Description
Boster Bio Anti-1433 Eta (eta) Monoclonal Antibody catalog # M04219. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Canine, Equine, Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-1433 Eta (eta) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04219)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length human recombinant 14.3.3 (eta) protein expressed in and purified from E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04219 is reactive to YWHAH in Bovine, Canine, Equine, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
28219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04219 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000. IF/ICC and IHC: 1:1,000.
Validation Images & Assay Conditions
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Western blot analysis of whole brain lysates (lanes 2,3), and cell lysates (lanes 4-8), using mouse mAb to 14.3.3, M04219, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells. Strong band at 28kDa corresponds to 14.3.3 protein, expressed in all preparations.
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to 14.3.3, M04219, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The M04219 antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.
Protein Target Info & Infographic
Gene/Protein Information For YWHAH (Source: Uniprot.org, NCBI)
Gene Name
YWHAH
Full Name
14-3-3 protein eta
Weight
28219 MW
Superfamily
14-3-3 family
Alternative Names
14-3-3 protein eta;Protein AS1;YWHAH;YWHA1; YWHAH YWHA1 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta 14-3-3 protein eta|14-3-3 eta|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YWHAH, check out the YWHAH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YWHAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-1433 Eta (eta) Monoclonal Antibody (M04219)
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No publications found for M04219
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Customer Q&As
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5 Customer Q&As for Anti-1433 Eta (eta) Monoclonal Antibody
Question
Do you have a BSA free version of anti-1433 Eta (eta) Monoclonal antibody M04219 available?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-1433 Eta (eta) Monoclonal antibody M04219 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-05
Question
Would anti-1433 Eta (eta) Monoclonal antibody M04219 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
According to the expression profile of leukemic t-cell, YWHAH is highly expressed in leukemic t-cell. So, it is likely that anti-1433 Eta (eta) Monoclonal antibody M04219 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-10-22
Question
We are currently using anti-1433 Eta (eta) Monoclonal antibody M04219 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says bovine, canine, equine, human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The anti-1433 Eta (eta) Monoclonal antibody (M04219) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-04
Question
I see that the anti-1433 Eta (eta) Monoclonal antibody M04219 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-1433 Eta (eta) Monoclonal antibody M04219. Let me know if you need anything else.
W. Jones
Verified customer
Asked: 2014-07-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-09
