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Product Info Summary
| SKU: | A30510 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-STEAP4/Stamp2 Antibody
View all STAMP2/STEAP4 Antibodies
SKU/Catalog Number
A30510
Size
100ul
Form
Liquid
Description
Boster Bio Anti-STEAP4/Stamp2 Antibody (Catalog# A30510). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STEAP4/Stamp2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30510)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human STEAP4. Immunogen sequence location: 201-250
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30510 is reactive to STEAP4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
51981 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30510 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using STEAP4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using STEAP4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For STEAP4 (Source: Uniprot.org, NCBI)
Gene Name
STEAP4
Full Name
Metalloreductase STEAP4
Weight
51981 MW
Superfamily
STEAP family
Alternative Names
STEAP4;STAMP2;TNFAIP9;Metalloreductase STEAP4;Six-transmembrane epithelial antigen of prostate 4;SixTransMembrane protein of prostate 2;Tumor necrosis factor; alpha-induced protein 9 STEAP4 STAMP2, SchLAH, TIARP, TNFAIP9 STEAP4 metalloreductase metalloreductase STEAP4|STEAP family member 4|seven chromosome locus associated with HCC|six transmembrane prostate protein 2|six-transmembrane epithelial of prostate 4|sixTransMembrane protein of prostate 2|tumor necrosis factor, alpha-induced protein 9|tumor necrosis-alpha-induced adipose-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STEAP4, check out the STEAP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STEAP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STEAP4/Stamp2 Antibody (A30510)
Hello CJ!
A30510 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and clinical significance of obesity-associated gene STEAP4 in obese children
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-STEAP4/Stamp2 Antibody
Question
Do you have a BSA free version of anti-STEAP4/Stamp2 antibody A30510 available?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STEAP4/Stamp2 antibody A30510 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-20
Question
We are currently using anti-STEAP4/Stamp2 antibody A30510 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-STEAP4/Stamp2 antibody (A30510) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
Can you help my question with product A30510, anti-STEAP4/Stamp2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30510 anti-STEAP4/Stamp2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
Will anti-STEAP4/Stamp2 antibody A30510 work for IHC with stomach?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
According to the expression profile of stomach, STEAP4 is highly expressed in stomach. So, it is likely that anti-STEAP4/Stamp2 antibody A30510 will work for IHC with stomach.
Boster Scientific Support
Answered: 2019-11-12
Question
I see that the anti-STEAP4/Stamp2 antibody A30510 works with IHC, what is the protocol used to produce the result images on the product page?
K. Kulkarni
Verified customer
Asked: 2018-12-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-31
Question
Would A30510 anti-STEAP4/Stamp2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Jha
Verified customer
Asked: 2017-12-04
Answer
It shows on the product datasheet, A30510 anti-STEAP4/Stamp2 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-04
