Product Info Summary
SKU: | A01501-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-PARN Antibody Picoband™
SKU/Catalog Number
A01501-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PARN Antibody Picoband™ catalog # A01501-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01501-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PARN recombinant protein (Position: M1-Y301).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01501-2 is reactive to PARN in Human, Mouse, Rat
Applications
A01501-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
78 kDa
Calculated molecular weight
Background of PARN
Poly (A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly (A) as the substrate, hence, efficiently degrades poly (A) tails of mRNAs. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
![A01501 2 PARN primary antibodies WB testing 1 A01501 2 PARN primary antibodies WB testing 1](https://www.bosterbio.com/media/catalog/product/A/0/A01501-2-PARN-primary-antibodies-WB-testing-1.jpg)
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Figure 1. Western blot analysis of PARN using anti-PARN antibody (A01501-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human PANC-1 whole cell lysates,
Lane 6: human SGC-7901 whole cell lysates,
Lane 7: human MDA-MB-231 whole cell lysates,
Lane 8: rat kidney tissue lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARN antigen affinity purified polyclonal antibody (Catalog # A01501-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARN at approximately 78KD. The expected band size for PARN is at 73KD.
![A01501 2 PARN primary antibodies IHC testing 2 A01501 2 PARN primary antibodies IHC testing 2](https://www.bosterbio.com/media/catalog/product/A/0/A01501-2-PARN-primary-antibodies-IHC-testing-2.jpg)
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Figure 2. IHC analysis of PARN using anti-PARN antibody (A01501-2).
PARN was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A01501 2 PARN primary antibodies IHC testing 3 A01501 2 PARN primary antibodies IHC testing 3](https://www.bosterbio.com/media/catalog/product/A/0/A01501-2-PARN-primary-antibodies-IHC-testing-3.jpg)
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Figure 3. IHC analysis of PARN using anti-PARN antibody (A01501-2).
PARN was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A01501 2 PARN primary antibodies IHC testing 4 A01501 2 PARN primary antibodies IHC testing 4](https://www.bosterbio.com/media/catalog/product/A/0/A01501-2-PARN-primary-antibodies-IHC-testing-4.jpg)
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Figure 4. IHC analysis of PARN using anti-PARN antibody (A01501-2).
PARN was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![a01501 2 parn primary antibodies if testing 5 a01501 2 parn primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a01501-2-parn-primary-antibodies-if-testing-5.png)
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Figure 5. IF analysis of PARN using anti-PARN antibody (A01501-2).
PARN was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a01501 2 parn primary antibodies fc testing 6 a01501 2 parn primary antibodies fc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a01501-2-parn-primary-antibodies-fc-testing-6.png)
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Figure 6. Flow Cytometry analysis of A431 cells using anti-PARN antibody (A01501-2).
Overlay histogram showing A431 cells stained with A01501-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARN Antibody (A01501-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PARN (Source: Uniprot.org, NCBI)
Gene Name
PARN
Full Name
Poly(A)-specific ribonuclease PARN
Weight
Superfamily
CAF1 family
Alternative Names
DANEC 3.1.13.4; Deadenylating nuclease; Deadenylation nuclease; poly(A)-specific ribonuclease (deadenylation nuclease); poly(A)-specific ribonuclease PARN; Polyadenylate-specific ribonuclease PARN DAN, DKCB6, PFBMFT4 poly(A)-specific ribonuclease poly(A)-specific ribonuclease PARN|deadenylating nuclease|deadenylation nuclease|polyadenylate-specific ribonuclease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARN, check out the PARN Infographic
![PARN infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PARN Antibody Picoband™ (A01501-2)
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