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Product Info Summary
| SKU: | M00523 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-STAT6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00523
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-STAT6 Rabbit Monoclonal Antibody catalog # M00523. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00523)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CFH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00523 is reactive to STAT6 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
94135 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00523 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Raji whole cell, human Hela whole cell, mouse thymus tissue
IHC: Rat stomach tissue, Human thyroid cancer tissue, Mouse lung tissue, rat liver tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STAT6 using anti-STAT6 antibody (M00523).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified polyclonal antibody (Catalog # M00523) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 100 kDa. The expected band size for STAT6 is at 94 kDa.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat liver, using STAT6 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:300 dilution.
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Immunofluorescent analysis of Hela cells, using STAT6 Antibody .
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
Protein Target Info & Infographic
Gene/Protein Information For STAT6 (Source: Uniprot.org, NCBI)
Gene Name
STAT6
Full Name
Signal transducer and activator of transcription 6
Weight
94135 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 6;IL-4 Stat;STAT6; STAT6 D12S1644, IL-4-STATB, STAT6C, STAT6 signal transducer and activator of transcription 6 signal transducer and activator of transcription 6|STAT, interleukin4-induced|signal transducer and activator of transcription 6, interleukin-4 induced|transcription factor IL-4 STAT
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT6, check out the STAT6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT6 Rabbit Monoclonal Antibody (M00523)
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Customer Q&As
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16 Customer Q&As for Anti-STAT6 Rabbit Monoclonal Antibody
Question
Our lab used your anti-STAT6 Rabbit Monoclonal antibody for IF on esophagus a few years ago. I am using human, and We want to use the antibody for IHC next. We need examining esophagus as well as tongue in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
I looked at the website and datasheets of our anti-STAT6 Rabbit Monoclonal antibody and it appears that M00523 has been tested on human in both IF and IHC. Thus M00523 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-STAT6 Rabbit Monoclonal antibody M00523 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-STAT6 Rabbit Monoclonal antibody (M00523) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
Is a blocking peptide available for product anti-STAT6 Rabbit Monoclonal antibody (M00523)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
We do provide the blocking peptide for product anti-STAT6 Rabbit Monoclonal antibody (M00523). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-02
Question
Is there a BSA free version of anti-STAT6 Rabbit Monoclonal antibody M00523 available?
S. Edwards
Verified customer
Asked: 2020-03-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STAT6 Rabbit Monoclonal antibody M00523 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-19
Question
Is this M00523 anti-STAT6 Rabbit Monoclonal antibody reactive to the isotypes of STAT6?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The immunogen of M00523 anti-STAT6 Rabbit Monoclonal antibody is A synthesized peptide derived from human STAT6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-24
Question
I see that the anti-STAT6 Rabbit Monoclonal antibody M00523 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-20
Question
Does anti-STAT6 Rabbit Monoclonal antibody M00523 work for IHC with esophagus?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
According to the expression profile of esophagus, STAT6 is highly expressed in esophagus. So, it is likely that anti-STAT6 Rabbit Monoclonal antibody M00523 will work for IHC with esophagus.
Boster Scientific Support
Answered: 2019-11-25
Question
Would M00523 anti-STAT6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Mangal
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, M00523 anti-STAT6 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-03
Question
I would like using your anti-STAT6 Rabbit Monoclonal antibody for t-helper 1 cell lineage commitment studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
Thank you for your inquiry. This M00523 anti-STAT6 Rabbit Monoclonal antibody is validated on raji cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-18
Question
you antibody to test anti-STAT6 Rabbit Monoclonal antibody M00523 on mouse esophagus for research purposes, then I may be interested in using anti-STAT6 Rabbit Monoclonal antibody M00523 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The products we sell, including anti-STAT6 Rabbit Monoclonal antibody M00523, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-18
Question
My boss were satisfied with the WB result of your anti-STAT6 Rabbit Monoclonal antibody. However we have been able to see positive staining in uterus cytoplasm. nucleus. note=translocated into using this antibody. Is that expected? Could you tell me where is STAT6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
According to literature, uterus does express STAT6. Generally STAT6 expresses in cytoplasm. nucleus. note=translocated into. Regarding which tissues have STAT6 expression, here are a few articles citing expression in various tissues:
Tongue, Pubmed ID: 14702039
Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-15
Question
My question regarding product M00523, anti-STAT6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Banerjee
Verified customer
Asked: 2019-03-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00523 anti-STAT6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-STAT6 Rabbit Monoclonal antibody M00523. Let me know if you need anything else.
C. Anderson
Verified customer
Asked: 2018-11-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-16
Question
I was wanting to use your anti-STAT6 Rabbit Monoclonal antibody for IHC for mouse esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse esophagus identification?
Verified Customer
Verified customer
Asked: 2017-12-08
Answer
You can see on the product datasheet, M00523 anti-STAT6 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-08
Question
See attached the WB image, lot number and protocol we used for esophagus using anti-STAT6 Rabbit Monoclonal antibody M00523. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-01
Question
We have observed staining in mouse esophagus. What should we do? Is anti-STAT6 Rabbit Monoclonal antibody supposed to stain esophagus positively?
T. Anderson
Verified customer
Asked: 2016-01-28
Answer
According to literature esophagus does express STAT6. According to Uniprot.org, STAT6 is expressed in esophagus, tongue, uterus, among other tissues. Regarding which tissues have STAT6 expression, here are a few articles citing expression in various tissues:
Tongue, Pubmed ID: 14702039
Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-01-28
