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Product Info Summary
| SKU: | PA1805 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband®
SKU/Catalog Number
PA1805
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Arginase-2, mitochondrial Arg2 Antibody catalog # PA1805. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1805)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Arg2, different from the related mouse sequence by three amino acids and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1805 is reactive to ARG2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
38578 MW
Background of ARG2
ARG2 (arginase, type II) encodes a 355-amino acid polypeptide. Using Northern blotting and RT-PCR, Vockley et al. (1996) found that ARG2 is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney. By PCR analysis of somatic cell hybrid panels, fluorescence in situ hybridization, and radiation hybrid analysis, the ARG2 gene is mapped to 14q24.1-q24.3. ARG2 may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al. (1996) showed that ARG2 mRNA and nitric oxide synthase (NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of ARG2 and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that ARG2 is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1805 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human 293T whole cell, human PC-3 whole cell, human K562 whole cell, human HepG2 whole cell
IHC: human prostate cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ARG2 using anti-ARG2 antibody (PA1805).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG2 antigen affinity purified polyclonal antibody (Catalog # PA1805) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG2 at approximately 38 kDa. The expected band size for ARG2 is at 39 kDa.
Click image to see more details
Figure 2. IHC analysis of ARG2 using anti-ARG2 antibody (PA1805).
ARG2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARG2 Antibody (PA1805) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ARG2 (Source: Uniprot.org, NCBI)
Gene Name
ARG2
Full Name
Arginase-2, mitochondrial
Weight
38578 MW
Superfamily
arginase family
Alternative Names
Arginase-2, mitochondrial;3.5.3.1;Kidney-type arginase;Non-hepatic arginase;Type II arginase;ARG2; ARG2 arginase 2 arginase-2, mitochondrial|L-arginine amidinohydrolase|L-arginine ureahydrolase|arginase II|arginase, type II|kidney arginase|kidney-type arginase|non-hepatic arginase|nonhepatic arginase|type II arginase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARG2, check out the ARG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband® (PA1805)
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Customer Q&As
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5 Customer Q&As for Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband®
Question
Will PA1805 have any cross reactivity to Arginase 1 (ARG1) which is highly expressed in the liver?
Verified customer
Asked: 2020-10-23
Answer
Our lab hasn't experimentally validated cross-reactivity with Arginase 1 (ARG1) using PA1805. The immunogen sequence of PA1805 is DFGDLSFTPVPKDDLYNNL which has very low sequence homology with Arginase 1 (ARG1).
Boster Scientific Support
Answered: 2020-10-25
Question
Here is the WB image, lot number and protocol we used for lung using anti-Arg2 antibody PA1805. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Arg2 antibody PA1805. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
We are currently using anti-Arg2 antibody PA1805 for rat tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
The anti-Arg2 antibody (PA1805) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-28
Question
Does PA1805 anti-Arg2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Jha
Verified customer
Asked: 2014-11-03
Answer
It shows on the product datasheet, PA1805 anti-Arg2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-03
