Click image to see more details
Product Info Summary
| SKU: | PA1841 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-STAT5b Antibody Picoband®
SKU/Catalog Number
PA1841
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STAT5b Antibody catalog # PA1841. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT5b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1841)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5b, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1841 is reactive to STAT5B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
89866 MW
Background of STAT5B
STAT5B (Signal transducer and activator of transcription 5B) is a protein that in humans is encoded by the STAT5B gene. STAT5B orthologs have been identified in most placentals for which complete genome data are available. By FISH, Lin et al. (1996) mapped STAT5B to 17q11.2. The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APML). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APML.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1841 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Intestine Tissue, Rat Kidney Tissue, HELA Cell, A549 Cell, MM231 Cell, COLO320 Cell,
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-STAT5b antibody, PA1841, Western blotting
Lane 1: Rat Intestine Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MM231 Cell Lysate
Lane 6: COLO320 Cell Lysate
Click image to see more details
Anti-STAT5b antibody, PA1841, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For STAT5B (Source: Uniprot.org, NCBI)
Gene Name
STAT5B
Full Name
Signal transducer and activator of transcription 5B
Weight
89866 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 5B;STAT5B; STAT5B GHISID2, STAT5 signal transducer and activator of transcription 5B signal transducer and activator of transcription 5B|transcription factor STAT5B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT5B, check out the STAT5B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT5b Antibody Picoband® (PA1841)
Hello CJ!
PA1841 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Severe growth failure associated with a novel heterozygous nonsense mutation in the GHR transmembrane domain leading to elevated growth hormone binding protein
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-STAT5b Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-STAT5b Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-STAT5b Antibody Picoband®
Question
I have a question about product PA1841, anti-STAT5b antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1841 anti-STAT5b antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-18
Question
Does PA1841 anti-STAT5b antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
As indicated on the product datasheet, PA1841 anti-STAT5b antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-17
Question
We want to test anti-STAT5b antibody PA1841 on rat lymph for research purposes, then I may be interested in using anti-STAT5b antibody PA1841 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The products we sell, including anti-STAT5b antibody PA1841, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-21
Question
Will anti-STAT5b antibody PA1841 work on pig WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Our lab technicians have not validated anti-STAT5b antibody PA1841 on pig. You can run a BLAST between pig and the immunogen sequence of anti-STAT5b antibody PA1841 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-31
Question
Is a blocking peptide available for product anti-STAT5b antibody (PA1841)?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
We do provide the blocking peptide for product anti-STAT5b antibody (PA1841). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-04
Question
Is there a BSA free version of anti-STAT5b antibody PA1841 available?
B. Parker
Verified customer
Asked: 2017-02-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STAT5b antibody PA1841 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-16
