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Key=created_at, value=2017-12-15 02:49:43
Key=updated_at, value=2024-09-26 00:55:47
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Key=name, value=Midkine Rat Recombinant Protein
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Key=description, value=Midkine Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2kDa. The Midkine Rat is purified by proprietary chromatographic techniques.
Key=short_description, value=Midkine Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2kDa. The Midkine Rat is purified by proprietary chromatographic techniques.
Key=meta_keyword, value=Midkine Rat Recombinant Protein, recombinant protein, cell culture, PROTQ9R1S9
Key=contents, value=Lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.
Key=gene_name, value=MDK
Key=synonyms, value=Midkine; MK; Mdk
Key=subcellular_localization, value= Secreted
Key=tissue_specificity, value= Expressed at a low level in arteries, and at higher levels in newly formed neointima. In brain, expressed in the caudate nucleus and the brain stem.
Key=protein_function, value= Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity).
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Amino Acid Sequence

VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD


Key=custom_listing_information, value=
Available Sizes:5ug, 20ug, 1mg
Applications:ex vivo Cell Culture
Source:E. Coli
Purity:Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Key=purification, value=Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Key=storage, value=Lyophilized Midkine Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine Rat should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
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Biological Activity

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.


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Key=meta_description, value=PROTQ9R1S9 Midkine Rat Recombinant Protein Midkine Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2kDa.
The Midkine Rat is purified by proprietary chromat
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Key=reconstitution, value=It is recommended to reconstitute the lyophilized Midkine Rat in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Key=reactivity, value=Rat
Key=form, value=Sterile Filtered White lyophilized (freeze-dried) powder.
Key=source, value=Escherichia coli
Key=source_company, value=ProSpec CYT-747; Cost: 5ug $45, 20ug $100, 1mg $1900
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Midkine Rat Recombinant Protein

Midkine protein, Rat

Midkine Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2kDa. The Midkine Rat is purified by proprietary chromatographic techniques.

Product Info Summary

SKU: PROTQ9R1S9
Size: 5ug, 20ug, 1mg
Origin Species: Rat
Source: Escherichia coli

Product Name

Midkine Rat Recombinant Protein

View all Midkine recombinant proteins

SKU/Catalog Number

PROTQ9R1S9

Size

5ug, 20ug, 1mg

Description

Midkine Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2kDa. The Midkine Rat is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Midkine Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine Rat should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Cite This Product

Midkine Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ9R1S9)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.

Purity

Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

15.585kDa

Reconstitution

It is recommended to reconstitute the lyophilized Midkine Rat in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Amino Acid Sequence

VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD

Biological Activity

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.

Reconstitution

It is recommended to reconstitute the lyophilized Midkine Rat in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For MDK (Source: Uniprot.org, NCBI)

Gene Name

MDK

Full Name

Midkine

Weight

15.585kDa

Superfamily

pleiotrophin family

Alternative Names

Midkine; MK; Mdk MDK ARAP, MK, NEGF2 midkine midkine|amphiregulin-associated protein|midgestation and kidney protein|neurite growth-promoting factor 2|neurite outgrowth-promoting factor 2|retinoic acid inducible factor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on MDK, check out the MDK Infographic

MDK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MDK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Total: $250

SKU:PROTQ9R1S9

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