Key=store_id
Key=entity_id, value=47481
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EK1711
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Key=required_options, value=0
Key=created_at, value=2018-05-31 13:07:48
Key=updated_at, value=2024-09-26 00:58:25
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Mouse Serpin A1 ELISA Kit PicoKine®
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Key=price, value=499.0000
Key=weight, value=1.0000
Key=meta_title, value=Mouse AAT ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Mouse Serpin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpina1c in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Key=image, value=/e/k/ek1711.png
Key=small_image, value=/e/k/ek1711.png
Key=thumbnail, value=/e/k/ek1711.png
Key=url_key, value=mouse-aat-picokine-trade-elisa-kit-ek1711-boster
Key=url_path, value=mouse-aat-picokine-trade-elisa-kit-ek1711-boster
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Key=image_label, value=Mouse AAT PicoKine ELISA Kit standard curve
Key=small_image_label, value=Mouse AAT PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Mouse AAT PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=gene_full_name, value=Alpha-1-antitrypsin 1-3
Key=uniprot_id, value=Q00896
Key=assay_range, value=1.56 ng/ml - 100 ng/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Mouse
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=100 ng/ml, 50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK1711
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Surface Molecules, Transcription, Transcription Factors, Transcription Factors/Regulators
Key=rating_value, value=98.9
Key=review_count, value=17
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Key=cj_related_products, value=Janiceps:A01073^Liver Regeneration:A01308|PA1124|PA1623|PB9141^Tissue Adhesions:PROTQ5JQS6
Key=description, value=Mouse Serpin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpina1c in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Mouse Serpin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpina1c in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=background, value=SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Key=gene_name, value=Serpina1c
Key=synonyms, value=Serpina1c; Dom3; Dom6; Spi1-3; Spi1-6; Alpha-1-antitrypsin 1-3; Alpha-1 protease inhibitor 3; Alpha-1 protease inhibitor 6; Alpha-1-antitrypsin 1-6; Serine protease inhibitor 1-3; Serine protease inhibitor 1-6; Serine protease inhibitor A1c; Serpin A1c
Key=sample_type, value=cell culture supernatants, serum and plasma (heparin, EDTA).
Key=kit_components, value=100 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2876	13987	37231	2911	14122	4269
Standard deviation	158.18	713.3	1600.9	177.57	932.05	320.18
CV (%)	5.5%	5.1%	4.3%	6.1%	6.6%	7.5%

Key=application_details, value=Dilution ratio of 1:10000, concentration in serum and plasma is 30-160 ug/ml.
Key=protein_function, value=Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.
Key=sample_data, value=

Concentration (ng/ml)	0	1.56	3.13	6.25	12.5	25	50	100
O.D.	0.042	0.207	0.368	0.659	1.054	1.369	1.645	2.069

Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: E25-K413
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2876	2791	2438	2616	2680	168.37	6.2%
Sample . 2	13987	13721	13461	14393	13890	344.60	2.4%%
Sample 3	37231	37794	38247	41965	38809	1857.18	4.7%

*number of samples for each test n=16.
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