Product Info Summary
SKU: | M00007-3 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7]
SKU/Catalog Number
M00007-3
Size
100 µl
Description
Boster Bio Anti-STAT3 mouse monoclonal antibody, clone OTI21E7 (formerly 21E7). Catalog# M00007-3. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00007-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI21E7
Isotype
IgG1
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 154-175 of human stat3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00007-3 is reactive to STAT3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
88.068kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00007-3 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-STAT3 (M00007-3) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and STAT3-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody M00007-3. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control (1:500).
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Western blot analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody (1:500).
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Western blot analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For STAT3 (Source: Uniprot.org, NCBI)
Gene Name
STAT3
Full Name
Signal transducer and activator of transcription 3
Weight
88.068kDa
Superfamily
transcription factor STAT family
Alternative Names
Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3 STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT3, check out the STAT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7] (M00007-3)
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