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Product Info Summary
| SKU: | A00036-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-STAT1 Antibody Picoband®
SKU/Catalog Number
A00036-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STAT1 Antibody Picoband® catalog # A00036-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00036-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00036-2 is reactive to STAT1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
91 kDa
Calculated molecular weight
87335 MW
Background of STAT1
Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00036-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human Caco-2 whole cell, human Raji whole cell
IHC: human intestinal cancer tissue
ICC/IF: U20S cell, U20S cell
FCM: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # A00036-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.
Click image to see more details
Figure 2. IHC analysis of STAT1 using anti-STAT1 antibody (A00036-2).
STAT1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STAT1 Antibody (A00036-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of STAT1 using anti-STAT1 antibody (A00036-2)
STAT1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-STAT1 Antibody (A00036-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 4. IF analysis of STAT1 using anti-STAT1 antibody (A00036-2)
STAT1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-STAT1 Antibody (A00036-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of A431 cells using anti-STAT1 antibody (A00036-2).
Overlay histogram showing A431 cells stained with A00036-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (A00036-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For STAT1 (Source: Uniprot.org, NCBI)
Gene Name
STAT1
Full Name
Signal transducer and activator of transcription 1-alpha/beta
Weight
87335 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 1-alpha/beta;Transcription factor ISGF-3 components p91/p84;STAT1; STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT1, check out the STAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT1 Antibody Picoband® (A00036-2)
Hello CJ!
A00036-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Synergistic inhibition of colon cancer growth by the combination of methylglyoxal and silencing of glyoxalase I mediated by the STAT1 pathway
Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
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Customer Q&As
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16 Customer Q&As for Anti-STAT1 Antibody Picoband®
Question
Do you have a BSA free version of anti-STAT1 antibody A00036-2 available?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-STAT1 antibody A00036-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-13
Question
My question regards to test anti-STAT1 antibody A00036-2 on monkey placenta testis for research purposes, then I may be interested in using anti-STAT1 antibody A00036-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The products we sell, including anti-STAT1 antibody A00036-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-06
Question
Our lab were content with the WB result of your anti-STAT1 antibody. However we have been able to see positive staining in cervix carcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
From what I have seen in literature, cervix carcinoma does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta testis using anti-STAT1 antibody A00036-2. Let me know if you need anything else.
L. Krishna
Verified customer
Asked: 2019-11-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
We are currently using anti-STAT1 antibody A00036-2 for monkey tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The anti-STAT1 antibody (A00036-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-17
Question
Would A00036-2 anti-STAT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
As indicated on the product datasheet, A00036-2 anti-STAT1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-30
Question
Can you help my question with product A00036-2, anti-STAT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Johnson
Verified customer
Asked: 2019-04-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00036-2 anti-STAT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-09
Question
I see that the anti-STAT1 antibody A00036-2 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-25
Question
Our lab want to know about using your anti-STAT1 antibody for response to mechanical stimulus studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
K. Parker
Verified customer
Asked: 2019-02-18
Answer
Thanks for your inquiry. This A00036-2 anti-STAT1 antibody is validated on human hela, hela whole cell lysates, a549 whole cell lysates, k562 whole cell lysates, a431 cells, rat thymus tissue, mouse thymus tissue. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-18
Question
Is a blocking peptide available for product anti-STAT1 antibody (A00036-2)?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
We do provide the blocking peptide for product anti-STAT1 antibody (A00036-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-07
Question
I was wanting to use your anti-STAT1 antibody for ICC for monkey placenta testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for monkey placenta testis identification?
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
As indicated on the product datasheet, A00036-2 anti-STAT1 antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in monkey placenta testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-06
Question
Here is the WB image, lot number and protocol we used for placenta testis using anti-STAT1 antibody A00036-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-21
Question
We have observed staining in monkey epithelium of bronchus. Any tips? Is anti-STAT1 antibody supposed to stain epithelium of bronchus positively?
G. Anderson
Verified customer
Asked: 2016-12-22
Answer
From what I have seen in literature epithelium of bronchus does express STAT1. From what I have seen in Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-12-22
Question
Is this A00036-2 anti-STAT1 antibody reactive to the isotypes of STAT1?
A. Huang
Verified customer
Asked: 2016-03-04
Answer
The immunogen of A00036-2 anti-STAT1 antibody is E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-03-04
Question
We have tried in the past anti-STAT1 antibody for ICC on leukemic t-cell in the past. I am using monkey, and I plan to use the antibody for Flow Cytometry next. We need examining leukemic t-cell as well as epithelium of bronchus in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
T. Yang
Verified customer
Asked: 2015-01-08
Answer
I have checked the website and datasheets of our anti-STAT1 antibody and it seems that A00036-2 has been validated on monkey in both ICC and Flow Cytometry. Thus A00036-2 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in monkey even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-08
Question
Would anti-STAT1 antibody A00036-2 work for ICC with placenta testis?
C. Anderson
Verified customer
Asked: 2014-08-25
Answer
According to the expression profile of placenta testis, STAT1 is highly expressed in placenta testis. So, it is likely that anti-STAT1 antibody A00036-2 will work for ICC with placenta testis.
Boster Scientific Support
Answered: 2014-08-25
