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Product Info Summary
| SKU: | PA1707 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SOCS3 Antibody Picoband®
SKU/Catalog Number
PA1707
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOCS3 Antibody catalog # PA1707. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOCS3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1707)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SOCS3, identical to the related mouse sequence, different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1707 is reactive to SOCS3 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
24770 MW
Background of SOCS-3
SOCS3 (Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1707 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: human Hela whole cell, human A549 whole cell, human A431 whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOCS3 using anti-SOCS3 antibody (PA1707).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (Catalog # PA1707) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 30KD. The expected band size for SOCS3 is at 25KD.
Click image to see more details
Figure 2. IHC analysis of SOCS3 using anti-SOCS3 antibody (PA1707).
SOCS3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOCS3 Antibody (PA1707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SOCS3 (Source: Uniprot.org, NCBI)
Gene Name
SOCS3
Full Name
Suppressor of cytokine signaling 3
Weight
24770 MW
Alternative Names
Suppressor of cytokine signaling 3;SOCS-3;Cytokine-inducible SH2 protein 3;CIS-3;STAT-induced STAT inhibitor 3;SSI-3;SOCS3;CIS3, SSI3; SOCS3 ATOD4, CIS3, Cish3, SOCS-3, SSI-3, SSI3 suppressor of cytokine signaling 3 suppressor of cytokine signaling 3|STAT-induced STAT inhibitor 3|cytokine-inducible SH2 protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOCS3, check out the SOCS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOCS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOCS3 Antibody Picoband® (PA1707)
Hello CJ!
PA1707 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang M, Tian M, Zhang X, Xu J, Yang B, Yu J, Li F, Li Y, Li S, Li X. Oncotarget. 2017 Jun 16;8(57):96958-96969. doi: 10.18632/oncotarget.18555. eCollection 2017 Nov 14. Role of the JAK2/STAT3 signaling pathway in the pathogenesis of type 2 diabete...
Wan J, Che Y, Kang N, Wu W. Mol Med Rep. 2015 Jul;12(1):83-92. Doi: 10.3892/Mmr.2015.3368. Epub 2015 Feb 17. Socs3 Blocks Hif-1?? Expression To Inhibit Proliferation And Angiogenesis Of Human Small Cell Lung Cancer By Downregulating Activation Of ...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-SOCS3 Antibody Picoband®
Question
We need to test anti-SOCS3 antibody PA1707 on human placenta for research purposes, then I may be interested in using anti-SOCS3 antibody PA1707 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The products we sell, including anti-SOCS3 antibody PA1707, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-13
Question
Does anti-SOCS3 antibody PA1707 work on primate IHC with skeletal muscle?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Our lab technicians have not validated anti-SOCS3 antibody PA1707 on primate. You can run a BLAST between primate and the immunogen sequence of anti-SOCS3 antibody PA1707 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skeletal muscle in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
We are currently using anti-SOCS3 antibody PA1707 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The anti-SOCS3 antibody (PA1707) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-11
Question
Does anti-SOCS3 antibody PA1707 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to the expression profile of placenta, SOCS3 is highly expressed in placenta. So, it is likely that anti-SOCS3 antibody PA1707 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-01-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-SOCS3 antibody PA1707. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-28
Question
Our lab used your anti-SOCS3 antibody for IHC on mucosa of stomach in a previous project. I am using human, and We intend to use the antibody for WB next. I am looking for examining mucosa of stomach as well as skeletal muscle in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I looked at the website and datasheets of our anti-SOCS3 antibody and it appears that PA1707 has been tested on human in both IHC and WB. Thus PA1707 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-21
Question
Is this PA1707 anti-SOCS3 antibody reactive to the isotypes of SOCS3?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The immunogen of PA1707 anti-SOCS3 antibody is A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-01
Question
We have seen staining in human skeletal muscle. Are there any suggestions? Is anti-SOCS3 antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
From literature skeletal muscle does express SOCS3. From Uniprot.org, SOCS3 is expressed in mucosa of stomach, t-cell lymphoma, skeletal muscle, placenta, among other tissues. Regarding which tissues have SOCS3 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Skeletal muscle, Pubmed ID: 11071852
T-cell lymphoma, Pubmed ID: 9266833
Boster Scientific Support
Answered: 2019-08-07
Question
Would PA1707 anti-SOCS3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Wu
Verified customer
Asked: 2019-01-30
Answer
You can see on the product datasheet, PA1707 anti-SOCS3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-30
Question
Is a blocking peptide available for product anti-SOCS3 antibody (PA1707)?
N. Jones
Verified customer
Asked: 2017-06-27
Answer
We do provide the blocking peptide for product anti-SOCS3 antibody (PA1707). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-27
Question
I was wanting to use your anti-SOCS3 antibody for WB for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
You can see on the product datasheet, PA1707 anti-SOCS3 antibody has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-12
Question
I see that the anti-SOCS3 antibody PA1707 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-02
Question
I am interested in using your anti-SOCS3 antibody for positive regulation of cell differentiation studies. Has this antibody been tested with western blotting on human hela? We would like to see some validation images before ordering.
Z. Zhang
Verified customer
Asked: 2016-11-03
Answer
Thanks for your inquiry. This PA1707 anti-SOCS3 antibody is validated on human hela, hela whole cell lysates, a549 whole cell lysates, a431 whole cell lysates. It is guaranteed to work for IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-03
Question
I have a question about product PA1707, anti-SOCS3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Mangal
Verified customer
Asked: 2014-07-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1707 anti-SOCS3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-09
Question
Here is the WB image, lot number and protocol we used for placenta using anti-SOCS3 antibody PA1707. Please let me know if you require anything else.
A. Evans
Verified customer
Asked: 2013-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-25
Question
Do you have a BSA free version of anti-SOCS3 antibody PA1707 available?
D. Collins
Verified customer
Asked: 2013-01-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SOCS3 antibody PA1707 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-04
