Product Info Summary
SKU: | A01678-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SREBP2/SREBF2 Antibody Picoband®
SKU/Catalog Number
A01678-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SREBP2/SREBF2 Antibody Picoband® catalog # A01678-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SREBP2/SREBF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01678-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SREBP2/SREBF2 recombinant protein (Position: R371-L409).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01678-2 is reactive to SREBF2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
50514 MW
Background of SREBP2
Sterol regulatory element-binding protein 2 (SREBP-2) also known as sterol regulatory element binding transcription factor 2 (SREBF2) is a protein that in humans is encoded by the SREBF2 gene. This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01678-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell, human Jurkat whole cell, human Raji whole cell, human HL-60 whole cell, rat stomach tissue, rat testis tissue, mouse stomach tissue, mouse testis tissue
ICC/IF: MCF-7 cell
FCM: K562 cell, RH35 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SREBP2/SREBF2 using anti-SREBP2/SREBF2 antibody (A01678-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: rat stomach tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse stomach tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SREBP2/SREBF2 antigen affinity purified polyclonal antibody (Catalog # A01678-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SREBP2/SREBF2 at approximately 68 kDa. The expected band size for SREBP2/SREBF2 is at 124 kDa.
Click image to see more details
Figure 2. IF analysis of SREBP2/SREBF2 using anti-SREBP2/SREBF2 antibody (A01678-2).
SREBP2/SREBF2 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SREBP2/SREBF2 Antibody (A01678-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of K562 cells using anti-SREBP2/SREBF2 antibody (A01678-2).
Overlay histogram showing K562 cells stained with A01678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SREBP2/SREBF2 Antibody (A01678-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 4. Flow Cytometry analysis of RH35 cells using anti-SREBP2/SREBF2 antibody (A01678-2).
Overlay histogram showing RH35 cells stained with A01678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SREBP2/SREBF2 Antibody (A01678-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SREBF2 (Source: Uniprot.org, NCBI)
Gene Name
SREBF2
Full Name
Sterol regulatory element-binding protein 2
Weight
50514 MW
Superfamily
SREBP family
Alternative Names
Gastrin/cholecystokinin type B receptor; CCK-B receptor; CCK-BR; Cholecystokinin-2 receptor; CCK2-R; CCKBR; CCKRB SREBF2 SREBP-2, SREBP2, bHLHd2 sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2|class D basic helix-loop-helix protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SREBF2, check out the SREBF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SREBF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SREBP2/SREBF2 Antibody Picoband® (A01678-2)
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