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Product Info Summary
| SKU: | PA1909 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
View all Cytochrome P450 2E1 Antibodies
SKU/Catalog Number
PA1909
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 2E1/CYP2E1 Antibody catalog # PA1909. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1909)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1909 is reactive to Cyp2e1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
56805 MW
Background of Cytochrome P450 2E1
CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1909 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat liver tissue, mouse liver tissue
IHC: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (PA1909).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (Catalog # PA1909) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.
Click image to see more details
Figure 2. IHC analysis of CYP2E using anti-CYP2E antibody (PA1909).
CYP2E was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E Antibody (PA1909) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CYP2E using anti-CYP2E antibody (PA1909).
CYP2E was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E Antibody (PA1909) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Cyp2e1 (Source: Uniprot.org, NCBI)
Gene Name
Cyp2e1
Full Name
Cytochrome P450 2E1
Weight
56805 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7;CYPIIE1;Cytochrome P450-ALC;Cytochrome P450-J;Cyp2e1;Cyp2e, Cyp2e-1; CYP2E1 CPE1, CYP2E, P450-J, P450C2E cytochrome P450 family 2 subfamily E member 1 cytochrome P450 2E1|4-nitrophenol 2-hydroxylase|CYPIIE1|cytochrome P450, family 2, subfamily E, polypeptide 1|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1|cytochrome P450-J|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cyp2e1, check out the Cyp2e1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cyp2e1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (PA1909)
Hello CJ!
PA1909 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Thida,M.,Li,B.,Zhang,X.,Chen,C.,& Zhang,X.(2021).Echinacoside alleviates acetaminophen-induced liver injury by attenuating oxidative stress and inflammatory cytokines in mice.J Appl Biomed,19(2),112.doi:10.32725/jab.2021.011
Species: Mouse
PA1909 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500
Zhai T,Xu W,Liu Y,Qian K,Xiong Y,Chen Y.Honokiol Alleviates Methionine-Choline Deficient Diet-Induced Hepatic Steatosis and Oxidative Stress in C57BL/6 Mice by Regulating CFLAR-JNK Pathway.Oxid Med Cell Longev.2020 Nov 27;2020:2313641.doi:10.1155/2020/231
Species: Mouse
PA1909 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Schisandra sphenanthera extract (Wuzhi Tablet) protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway %u2026
Chen X, Sun Ck, Han Gz, Peng Jy, Li Y, Liu Yx, Lv Yy, Liu Kx, Zhou Q, Sun Hj. World J Gastroenterol. 2009 Apr 21;15(15):1829-35. Protective Effect Of Tea Polyphenols Against Paracetamol-Induced Hepatotoxicity In Mice Is Significantly Correlated Wi...
Fan X, Jiang Y, Wang Y, Tan H, Zeng H, Wang Y, Chen P, Qu A, Gonzalez Fj, Huang M, Bi H. Drug Metab Dispos. 2014 Dec;42(12):1982-90. Doi: 10.1124/Dmd.114.059535. Epub 2014 Sep 12. Wuzhi Tablet (Schisandra Sphenanthera Extract) Protects Against Ace...
Jiang Y, Fan X, Wang Y, Chen P, Zeng H, Tan H, Gonzalez Fj, Huang M, Bi H. Toxicol Sci. 2015 Jan;143(1):107-15. Doi: 10.1093/Toxsci/Kfu216. Epub 2014 Oct 14. Schisandrol B Protects Against Acetaminophen-Induced Hepatotoxicity By Inhibition Of Cyp-...
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Customer Q&As
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17 Customer Q&As for Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
Question
Do you have a BSA free version of anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 available?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-22
Question
Is this PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody reactive to the isotypes of CYP2E1?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The immunogen of PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1 (94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-11
Question
We are currently using anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The anti-Cytochrome P450 2E1/CYP2E1 antibody (PA1909) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-27
Question
Would anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 work on goat WB with liver?
D. Miller
Verified customer
Asked: 2020-02-26
Answer
Our lab technicians have not validated anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-26
Question
Please see the WB image, lot number and protocol we used for liver using anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-17
Question
My question regarding product PA1909, anti-Cytochrome P450 2E1/CYP2E1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
I would like using your anti-Cytochrome P450 2E1/CYP2E1 antibody for exogenous drug catabolic process studies. Has this antibody been tested with western blotting on rat liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Thanks for your inquiry. This PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody is tested on rat liver tissue, mouse liver tissue. It is guaranteed to work for IHC-P, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-21
Question
We have observed staining in mouse brain. What should we do? Is anti-Cytochrome P450 2E1/CYP2E1 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Based on literature brain does express CYP2E1. Based on Uniprot.org, CYP2E1 is expressed in liver, brain, among other tissues. Regarding which tissues have CYP2E1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 3675576, 24275569
Boster Scientific Support
Answered: 2019-10-23
Question
I was wanting to use your anti-Cytochrome P450 2E1/CYP2E1 antibody for IHC-P for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
It shows on the product datasheet, PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody has been validated for IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-10
Question
I am interested in to test anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 on mouse liver for research purposes, then I may be interested in using anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The products we sell, including anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-06
Question
We ordered your anti-Cytochrome P450 2E1/CYP2E1 antibody for WB on liver in the past. I am using rat, and We are going to use the antibody for IHC-P next. We want examining liver as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
I took a look at the website and datasheets of our anti-Cytochrome P450 2E1/CYP2E1 antibody and it seems that PA1909 has been tested on rat in both WB and IHC-P. Thus PA1909 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-27
Question
Will PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
As indicated on the product datasheet, PA1909 anti-Cytochrome P450 2E1/CYP2E1 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-23
Question
Would anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 work for IHC-P with liver?
L. Yang
Verified customer
Asked: 2018-04-13
Answer
According to the expression profile of liver, CYP2E1 is highly expressed in liver. So, it is likely that anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 will work for IHC-P with liver.
Boster Scientific Support
Answered: 2018-04-13
Question
Our team were content with the WB result of your anti-Cytochrome P450 2E1/CYP2E1 antibody. However we have observed positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is CYP2E1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
From literature, liver does express CYP2E1. Generally CYP2E1 expresses in endoplasmic reticulum membrane. Regarding which tissues have CYP2E1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 3675576, 24275569
Boster Scientific Support
Answered: 2018-01-08
Question
I see that the anti-Cytochrome P450 2E1/CYP2E1 antibody PA1909 works with IHC-P, what is the protocol used to produce the result images on the product page?
K. Li
Verified customer
Asked: 2013-02-22
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-02-22
Question
Is a blocking peptide available for product anti-Cytochrome P450 2E1/CYP2E1 antibody (PA1909)?
C. Krishna
Verified customer
Asked: 2013-02-13
Answer
We do provide the blocking peptide for product anti-Cytochrome P450 2E1/CYP2E1 antibody (PA1909). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-02-13
