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Product Info Summary
| SKU: | A03117 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Bradykinin Receptor B2 Antibody
View all Bradykinin RB2/BDKRB2 Antibodies
SKU/Catalog Number
A03117
Size
100µg
Form
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Description
Boster Bio Anti-Bradykinin Receptor B2 Antibody catalog # A03117. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bradykinin Receptor B2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03117)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of rat bradykinin receptor B2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03117 is reactive to Bdkrb2 in Mouse, Rat
Reconstitution
Calculated molecular weight
132375 MW
Background of Bradykinin RB2/BDKRB2
FANCC (also called Protein FACC or Fanconi Anemia Group C protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCC may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCC belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is ubiquitously expressed. Defects in FANCC are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group C.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03117 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Membrane ELISA: Lewis rat heart membranes (5µ
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Figure 1. Western blot analysis of Bdkrb2 using anti-Bdkrb2 antibody (A03117).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bdkrb2 antigen affinity purified polyclonal antibody (Catalog # A03117) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Bdkrb2.
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Figure 2. IHC analysis of Bdkrb2 using anti-Bdkrb2 antibody (A03117).
Bdkrb2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bdkrb2 Antibody (A03117) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Bdkrb2 (Source: Uniprot.org, NCBI)
Gene Name
Bdkrb2
Full Name
B2 bradykinin receptor
Weight
132375 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
AT-rich interactive domain-containing protein 5B;ARID domain-containing protein 5B;MRF1-like protein;Modulator recognition factor 2;MRF-2;ARID5B;DESRT, MRF2; BDKRB2 B2R, BK-2, BK2, BKR2, BRB2 bradykinin receptor B2 B2 bradykinin receptor|BK-2 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Bdkrb2, check out the Bdkrb2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bdkrb2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bradykinin Receptor B2 Antibody (A03117)
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