Anti-Bradykinin Receptor B₂ Antibody

Product Name

Anti-Bradykinin Receptor B₂ Antibody

View all Bradykinin RB2/BDKRB2 Antibodies

SKU/Catalog Number

A03117

Size

100µg

Form

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Description

Boster Bio Anti-Bradykinin Receptor B₂ Antibody catalog # A03117. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bradykinin Receptor B₂ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03117)

Host

Rabbit

Contents

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus of rat bradykinin receptor B₂.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03117 is reactive to BDKRB2 in Mouse, Rat

Applications

A03117 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

132375 MW

Background of Bradykinin RB2/BDKRB2

FANCC (also called Protein FACC or Fanconi Anemia Group C protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCC may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCC belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is ubiquitously expressed. Defects in FANCC are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group C.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Click image to see more details

Membrane ELISA: Lewis rat heart membranes (5µ

Click image to see more details

Figure 1. Western blot analysis of Bdkrb2 using anti-Bdkrb2 antibody (A03117).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bdkrb2 antigen affinity purified polyclonal antibody (Catalog # A03117) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Bdkrb2.

Click image to see more details

Figure 2. IHC analysis of Bdkrb2 using anti-Bdkrb2 antibody (A03117).
Bdkrb2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bdkrb2 Antibody (A03117) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For BDKRB2 (Source: Uniprot.org, NCBI)

Alternative Names

B2 bradykinin receptor; B2R; BDKRB2; BK-2 receptor; BK2; BK-2; BKR2; Bradykinin RB2; bradykinin receptor B2; BradykininRB2; BRB2; DKFZp686O088 BDKRB2 B2R, BK-2, BK2, BKR2, BRB2 bradykinin receptor B2 B2 bradykinin receptor|BK-2 receptor

For more info on BDKRB2, check out the BDKRB2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BDKRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the BDKRB2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis