Click image to see more details
Product Info Summary
| SKU: | A00416-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SPR Antibody Picoband®
SKU/Catalog Number
A00416-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPR Antibody Picoband® catalog # A00416-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00416-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SPR recombinant protein (Position: V36-K261).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00416-1 is reactive to SPR in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.048kDa
Background of SPR
Sepiapterin reductase is an enzyme that in humans is encoded by the SPR gene. This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00416-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
ELISA,0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SPR using anti-SPR antibody (A00416-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPR antigen affinity purified polyclonal antibody (Catalog # A00416-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPR at approximately 28KD. The expected band size for SPR is at 28KD.
Protein Target Info & Infographic
Gene/Protein Information For SPR (Source: Uniprot.org, NCBI)
Gene Name
SPR
Full Name
Sepiapterin reductase
Weight
28.048kDa
Superfamily
sepiapterin reductase family
Alternative Names
Sepiapterin reductase; SPR; SPR SPR SDR38C1 sepiapterin reductase sepiapterin reductase|Sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)|sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)|short chain dehydrogenase/reductase family 38C, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPR, check out the SPR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPR Antibody Picoband® (A00416-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SPR Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SPR Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-SPR Antibody Picoband®
Question
See below the WB image, lot number and protocol we used for mucosa of transverse colon using anti-SPR antibody A00416-1. Please let me know if you require anything else.
R. Huang
Verified customer
Asked: 2020-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
Would A00416-1 anti-SPR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
It shows on the product datasheet, A00416-1 anti-SPR antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
I see that the anti-SPR antibody A00416-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-06
Question
Can you help my question with product A00416-1, anti-SPR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00416-1 anti-SPR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-12
Question
I am looking for to test anti-SPR antibody A00416-1 on human mucosa of transverse colon for research purposes, then I may be interested in using anti-SPR antibody A00416-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-SPR antibody A00416-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
My colleagues were happy with the WB result of your anti-SPR antibody. However we have seen positive staining in liver cytoplasm. using this antibody. Is that expected? Could you tell me where is SPR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
From literature, liver does express SPR. Generally SPR expresses in cytoplasm. Regarding which tissues have SPR expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15815621
Liver, Pubmed ID: 1883349, 24275569
Pancreas, Pubmed ID: 15489334
Salivary gland, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of transverse colon using anti-SPR antibody A00416-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-14
Question
Our lab want to know about using your anti-SPR antibody for salvage and regulation studies. Has this antibody been tested with western blotting on human hepg2 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
Thank you for your inquiry. This A00416-1 anti-SPR antibody is tested on human hepg2 whole cell lysates. It is guaranteed to work for ELISA, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-06
Question
I was wanting to use your anti-SPR antibody for WB for human mucosa of transverse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mucosa of transverse colon identification?
J. Parker
Verified customer
Asked: 2018-04-27
Answer
As indicated on the product datasheet, A00416-1 anti-SPR antibody has been validated for ELISA, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-27
Question
Will anti-SPR antibody A00416-1 work for WB with mucosa of transverse colon?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
According to the expression profile of mucosa of transverse colon, SPR is highly expressed in mucosa of transverse colon. So, it is likely that anti-SPR antibody A00416-1 will work for WB with mucosa of transverse colon.
Boster Scientific Support
Answered: 2018-01-31
Question
Is a blocking peptide available for product anti-SPR antibody (A00416-1)?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
We do provide the blocking peptide for product anti-SPR antibody (A00416-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-30
Question
Is this A00416-1 anti-SPR antibody reactive to the isotypes of SPR?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
The immunogen of A00416-1 anti-SPR antibody is E. coli-derived human SPR recombinant protein (Position: V36-K261). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-25
Question
Our lab used your anti-SPR antibody for WB on kidney in the past. I am using human, and We intend to use the antibody for ELISA next. Our lab want to know about examining kidney as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
R. Huang
Verified customer
Asked: 2016-06-23
Answer
I took a look at the website and datasheets of our anti-SPR antibody and I see that A00416-1 has been tested on human in both WB and ELISA. Thus A00416-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-06-23
Question
We have observed staining in mouse kidney. What should we do? Is anti-SPR antibody supposed to stain kidney positively?
S. Walker
Verified customer
Asked: 2015-11-10
Answer
According to literature kidney does express SPR. According to Uniprot.org, SPR is expressed in mucosa of transverse colon, liver, salivary gland, kidney, pancreas, erythroleukemia, among other tissues. Regarding which tissues have SPR expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15815621
Liver, Pubmed ID: 1883349, 24275569
Pancreas, Pubmed ID: 15489334
Salivary gland, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-11-10
Question
We are currently using anti-SPR antibody A00416-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
J. Krishna
Verified customer
Asked: 2015-11-09
Answer
The anti-SPR antibody (A00416-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-09
Question
Is there a BSA free version of anti-SPR antibody A00416-1 available?
L. Thomas
Verified customer
Asked: 2015-01-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SPR antibody A00416-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-01-12
