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Product Info Summary
| SKU: | PB9853 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Ceruloplasmin/CP Antibody Picoband®
View all Ceruloplasmin Antibodies
SKU/Catalog Number
PB9853
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ceruloplasmin/CP Antibody Picoband® catalog # PB9853. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ceruloplasmin/CP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9853)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Ceruloplasmin recombinant protein (Position: R20-M258). Mouse Ceruloplasmin shares 80.8% and 91.2% amino acid (aa) sequence identity with human and rat Ceruloplasmin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9853 is reactive to Cp in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
121151 MW
Background of Ceruloplasmin
Ceruloplasmin (or caeruloplasmin) is a ferroxidase enzyme that in humans is encoded by the CP gene. It is mapped to 3q23-q25. The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe (II)transferrin to Fe (III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9853 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Positive Control
WB: rat liver tissue, mouse liver tissue, mouse HEPA1-6 whole cell
IHC: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin/CP antigen affinity purified polyclonal antibody (Catalog # PB9853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ceruloplasmin/CP at approximately 130 kDa. The expected band size for Ceruloplasmin/CP is at 121 kDa.
Click image to see more details
Figure 2. IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853).
Ceruloplasmin/CP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ceruloplasmin/CP Antibody (PB9853) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853).
Ceruloplasmin/CP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ceruloplasmin/CP Antibody (PB9853) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Cp (Source: Uniprot.org, NCBI)
Gene Name
Cp
Full Name
Ceruloplasmin
Weight
121151 MW
Superfamily
multicopper oxidase family
Alternative Names
Ceruloplasmin;1.16.3.1;Ferroxidase;Cp; CP CP-2 ceruloplasmin ceruloplasmin|ceruloplasmin (ferroxidase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cp, check out the Cp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ceruloplasmin/CP Antibody Picoband® (PB9853)
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Customer Q&As
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17 Customer Q&As for Anti-Ceruloplasmin/CP Antibody Picoband®
Question
We ordered your anti-Ceruloplasmin/CP antibody for WB on cerebrospinal fluid last year. I am using rat, and We intend to use the antibody for IHC-P next. you antibody examining cerebrospinal fluid as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
I took a look at the website and datasheets of our anti-Ceruloplasmin/CP antibody and I see that PB9853 has been tested on rat in both WB and IHC-P. Thus PB9853 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-03
Question
Is this PB9853 anti-Ceruloplasmin/CP antibody reactive to the isotypes of CP?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The immunogen of PB9853 anti-Ceruloplasmin/CP antibody is E. coli-derived mouse Ceruloplasmin recombinant protein (Position: R20-M258). Mouse Ceruloplasmin shares 80.8% and 91.2% amino acid (aa) sequence identity with human and rat Ceruloplasmin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
I am interested in using your anti-Ceruloplasmin/CP antibody for cellular iron ion homeostasis studies. Has this antibody been tested with western blotting on mouse liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Thank you for your inquiry. This PB9853 anti-Ceruloplasmin/CP antibody is validated on rat liver tissue, mouse liver tissue. It is guaranteed to work for IHC-P, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-20
Question
Does anti-Ceruloplasmin/CP antibody PB9853 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
According to the expression profile of plasma, CP is highly expressed in plasma. So, it is likely that anti-Ceruloplasmin/CP antibody PB9853 will work for WB with plasma.
Boster Scientific Support
Answered: 2020-01-22
Question
Do you have a BSA free version of anti-Ceruloplasmin/CP antibody PB9853 available?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Ceruloplasmin/CP antibody PB9853 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-29
Question
I was wanting to use your anti-Ceruloplasmin/CP antibody for WB for mouse plasma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse plasma identification?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can see on the product datasheet, PB9853 anti-Ceruloplasmin/CP antibody has been validated for IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-15
Question
We were satisfied with the WB result of your anti-Ceruloplasmin/CP antibody. However we have been able to see positive staining in liver secreted. note=colocalizes with gcp1 in using this antibody. Is that expected? Could you tell me where is CP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
According to literature, liver does express CP. Generally CP expresses in secreted. note=colocalizes with gcp1 in. Regarding which tissues have CP expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Boster Scientific Support
Answered: 2019-11-05
Question
Can you help my question with product PB9853, anti-Ceruloplasmin/CP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9853 anti-Ceruloplasmin/CP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-30
Question
Please see the WB image, lot number and protocol we used for plasma using anti-Ceruloplasmin/CP antibody PB9853. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-02
Question
I see that the anti-Ceruloplasmin/CP antibody PB9853 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-03
Question
Will PB9853 anti-Ceruloplasmin/CP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
As indicated on the product datasheet, PB9853 anti-Ceruloplasmin/CP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
Will anti-Ceruloplasmin/CP antibody PB9853 work on dog IHC-P with plasma?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
Our lab technicians have not tested anti-Ceruloplasmin/CP antibody PB9853 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Ceruloplasmin/CP antibody PB9853 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-06
Question
I am interested in to test anti-Ceruloplasmin/CP antibody PB9853 on mouse plasma for research purposes, then I may be interested in using anti-Ceruloplasmin/CP antibody PB9853 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
The products we sell, including anti-Ceruloplasmin/CP antibody PB9853, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Ceruloplasmin/CP antibody PB9853. Let me know if you need anything else.
T. Jones
Verified customer
Asked: 2016-12-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-28
Question
We have observed staining in rat plasma. Do you have any suggestions? Is anti-Ceruloplasmin/CP antibody supposed to stain plasma positively?
R. Jha
Verified customer
Asked: 2016-08-04
Answer
From literature plasma does express CP. From Uniprot.org, CP is expressed in liver, bile, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have CP expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Boster Scientific Support
Answered: 2016-08-04
Question
Is a blocking peptide available for product anti-Ceruloplasmin/CP antibody (PB9853)?
B. Yang
Verified customer
Asked: 2015-09-15
Answer
We do provide the blocking peptide for product anti-Ceruloplasmin/CP antibody (PB9853). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-15
Question
We are currently using anti-Ceruloplasmin/CP antibody PB9853 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on horse tissues as well?
W. Krishna
Verified customer
Asked: 2015-01-20
Answer
The anti-Ceruloplasmin/CP antibody (PB9853) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-20
