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Product Info Summary
| SKU: | A13010 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Forkhead box protein D4 FOXD4 Antibody
SKU/Catalog Number
A13010
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein D4 FOXD4 Antibody (Catalog # A13010). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein D4 FOXD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13010)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human FOXD4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13010 is reactive to FOXD4 in Human, Mouse
Reconstitution
Calculated molecular weight
47309 MW
Background of FOXD4
This gene encodes a member of the forkhead/winged helix-box (FOX) family of transcription factors. FOX transcription factors play critical roles in the regulation of multiple processes including metabolism, cell proliferation and gene expression during ontogenesis. Mutations in this gene are associated with a complex phenotype consisting of dilated cardiomyopathy, obsessive-compulsive disorders, and suicidality.
Freyaldenhoven B.S., Gene 294:131-140(2002).
Humphray S.J., Nature 429:369-374(2004).
Fan Y., Genome Res. 12:1663-1672(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13010 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from NIH-3T3 cells, using FOXD4 antibody A13010.
Protein Target Info & Infographic
Gene/Protein Information For FOXD4 (Source: Uniprot.org, NCBI)
Gene Name
FOXD4
Full Name
Forkhead box protein D4
Weight
47309 MW
Alternative Names
Forkhead box protein D4;Forkhead-related protein FKHL9;Forkhead-related transcription factor 5;FREAC-5;Myeloid factor-alpha;FOXD4;FKHL9, FOXD4A, FREAC5; FOXD4 FKHL9A, FREAC-5, FREAC5, FOXD4 forkhead box D4 forkhead box protein D4|forkhead, Drosophila, homolog-like 9|forkhead-related activator 5|forkhead-related protein FKHL9|forkhead-related transcription factor 5|myeloid factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXD4, check out the FOXD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Forkhead box protein D4 FOXD4 Antibody (A13010)
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No publications found for A13010
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Customer Q&As
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6 Customer Q&As for Anti-Forkhead box protein D4 FOXD4 Antibody
Question
I see that the anti-FOXD4 antibody A13010 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-27
Question
We are currently using anti-FOXD4 antibody A13010 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The anti-FOXD4 antibody (A13010) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
Do you have a BSA free version of anti-FOXD4 antibody A13010 available?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXD4 antibody A13010 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-21
Question
I was wanting to use your anti-FOXD4 antibody for WB for human heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human heart identification?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
It shows on the product datasheet, A13010 anti-FOXD4 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-16
Question
Is this A13010 anti-FOXD4 antibody reactive to the isotypes of FOXD4?
J. Krishna
Verified customer
Asked: 2017-08-25
Answer
The immunogen of A13010 anti-FOXD4 antibody is Synthesized peptide derived from Internal of human FOXD4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-25
Question
My question regarding product A13010, anti-FOXD4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Johnson
Verified customer
Asked: 2014-05-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13010 anti-FOXD4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-13
