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Product Info Summary
| SKU: | A13433 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SPIC Antibody
SKU/Catalog Number
A13433
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SPIC Antibody (Catalog # A13433). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPIC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13433)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SPIC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13433 is reactive to SPIC in Human
Reconstitution
Calculated molecular weight
29180 MW
Background of Spic
Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression By similarity.
Carlsson R., Gene 299:271-278(2002).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13433 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells and HeLa cells, using SPIC antibody A13433.
Protein Target Info & Infographic
Gene/Protein Information For SPIC (Source: Uniprot.org, NCBI)
Gene Name
SPIC
Full Name
Transcription factor Spi-C
Weight
29180 MW
Superfamily
ETS family
Alternative Names
Transcription factor Spi-C;SPIC; Spic|AU019198, C76795, P, Prf, Sp, Spi-C|Spi-C transcription factor (Spi-1/PU.1 related)|transcription factor Spi-C|pu.1-related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPIC, check out the SPIC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPIC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPIC Antibody (A13433)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SPIC Antibody
Question
you antibody to test anti-SPIC antibody A13433 on human pancreas for research purposes, then I may be interested in using anti-SPIC antibody A13433 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Krishna
Verified customer
Asked: 2020-02-18
Answer
The products we sell, including anti-SPIC antibody A13433, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-18
Question
Does A13433 anti-SPIC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
As indicated on the product datasheet, A13433 anti-SPIC antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-28
Question
Is this A13433 anti-SPIC antibody reactive to the isotypes of SPIC?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
The immunogen of A13433 anti-SPIC antibody is Synthesized peptide derived from internal of human SPIC. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-13
Question
We are currently using anti-SPIC antibody A13433 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The anti-SPIC antibody (A13433) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-SPIC antibody A13433. Let me know if you need anything else.
H. Rodriguez
Verified customer
Asked: 2018-02-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-15
