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Product Info Summary
| SKU: | PB9618 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SOX4 Antibody Picoband®
SKU/Catalog Number
PB9618
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOX4 Antibody Picoband® catalog # PB9618. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9618)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SOX4, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9618 is reactive to SOX4 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
47263 MW
Background of SOX4
Transcription factor SOX-4 is a protein that in humans is encoded by the SOX4 gene. This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). This protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9618 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: MCF-7 Whole Cell, 293T Whole Cell, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate,
Lane 2: 293T Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
Click image to see more details
Figure 2. Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
Protein Target Info & Infographic
Gene/Protein Information For SOX4 (Source: Uniprot.org, NCBI)
Gene Name
SOX4
Full Name
Transcription factor SOX-4
Weight
47263 MW
Alternative Names
Transcription factor SOX-4;SOX4; SOX4 CSS10, EVI16 SRY-box transcription factor 4 transcription factor SOX-4|SRY (sex determining region Y)-box 4|SRY-box 4|SRY-related HMG-box gene 4|ecotropic viral integration site 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX4, check out the SOX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX4 Antibody Picoband® (PB9618)
Hello CJ!
PB9618 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huang J,Lu D,Xiang T,Wu X,Ge S,Wang Y,Wang J,Cheng N. MicroRNA-132-3p regulates cell proliferation, apoptosis, migration and invasion of liver cancer by targeting Sox4. Oncol Lett.2020 Apr;19(4):3173-3180.doi:10.3892/ol.2020.11431.Epub 2020 Mar 3.PMID:322
Species: Human
PB9618 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:1:500
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-SOX4 Antibody Picoband®
Question
Is a blocking peptide available for product anti-SOX4 antibody (PB9618)?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
We do provide the blocking peptide for product anti-SOX4 antibody (PB9618). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-04
Question
Would PB9618 anti-SOX4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can see on the product datasheet, PB9618 anti-SOX4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-SOX4 antibody PB9618. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
My colleagues were satisfied with the WB result of your anti-SOX4 antibody. However we have been able to see positive staining in cerebral cortex nucleus using this antibody. Is that expected? Could you tell me where is SOX4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
From what I have seen in literature, cerebral cortex does express SOX4. Generally SOX4 expresses in nucleus. Regarding which tissues have SOX4 expression, here are a few articles citing expression in various tissues:
Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-25
Question
Here is the WB image, lot number and protocol we used for ovary using anti-SOX4 antibody PB9618. Please let me know if you require anything else.
T. Jackson
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
I have a question about product PB9618, anti-SOX4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9618 anti-SOX4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-01
Question
Is there a BSA free version of anti-SOX4 antibody PB9618 available?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SOX4 antibody PB9618 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this PB9618 anti-SOX4 antibody reactive to the isotypes of SOX4?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
The immunogen of PB9618 anti-SOX4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SOX4 (1-31aa MVQQTNNAENTEALLAGESSDSGAGLELGIA), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-18
Question
We have been able to see staining in mouse cerebral cortex. Do you have any suggestions? Is anti-SOX4 antibody supposed to stain cerebral cortex positively?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
From literature cerebral cortex does express SOX4. From Uniprot.org, SOX4 is expressed in cerebral cortex, ovary, among other tissues. Regarding which tissues have SOX4 expression, here are a few articles citing expression in various tissues:
Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-04-16
Question
I see that the anti-SOX4 antibody PB9618 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-23
Question
We are currently using anti-SOX4 antibody PB9618 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
The anti-SOX4 antibody (PB9618) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-29
Question
Would anti-SOX4 antibody PB9618 work for WB with ovary?
K. Moore
Verified customer
Asked: 2017-10-12
Answer
According to the expression profile of ovary, SOX4 is highly expressed in ovary. So, it is likely that anti-SOX4 antibody PB9618 will work for WB with ovary.
Boster Scientific Support
Answered: 2017-10-12
Question
I was wanting to use your anti-SOX4 antibody for WB for human ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovary identification?
M. Jackson
Verified customer
Asked: 2017-09-29
Answer
As indicated on the product datasheet, PB9618 anti-SOX4 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-29
Question
I was wanting to use to test anti-SOX4 antibody PB9618 on human ovary for research purposes, then I may be interested in using anti-SOX4 antibody PB9618 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2015-02-27
Answer
The products we sell, including anti-SOX4 antibody PB9618, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-27
Question
I am interested in using your anti-SOX4 antibody for pro-b cell differentiation studies. Has this antibody been tested with western blotting on 293t whole cell lysate? We would like to see some validation images before ordering.
M. Huang
Verified customer
Asked: 2014-06-13
Answer
We appreciate your inquiry. This PB9618 anti-SOX4 antibody is validated on 293t whole cell lysate, mouse brain, brain tissue. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-06-13
