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Product Info Summary
| SKU: | M04004 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SOX18 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04004
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SOX18 Rabbit Monoclonal Antibody catalog # M04004. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX18 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04004)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DCD-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX18
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04004 is reactive to SOX18 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
41 kDa
Calculated molecular weight
40891 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04004 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human K562 whole cell, human COLO 320 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOX18 using anti-SOX18 antibody (M04004).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human COLO 320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX18 antigen affinity purified monoclonal antibody (Catalog # M04004) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX18 at approximately 41 kDa. The expected band size for SOX18 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SOX18 (Source: Uniprot.org, NCBI)
Gene Name
SOX18
Full Name
Transcription factor SOX-18
Weight
40891 MW
Alternative Names
Transcription factor SOX-18;SOX18; Sox18|AI385749, R, Ra, Ragl|SRY (sex determining region Y)-box 18|transcription factor SOX-18|SRY-box containing gene 18|Sry-related HMG-box gene 18|ragged
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX18, check out the SOX18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX18 Rabbit Monoclonal Antibody (M04004)
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1 Customer Q&As for Anti-SOX18 Rabbit Monoclonal Antibody
Question
We are currently using anti-SOX18 Rabbit Monoclonal antibody M04004 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-SOX18 Rabbit Monoclonal antibody (M04004) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
