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Product Info Summary
| SKU: | A01879-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human PDCD10 DyLight® 550 conjugated Antibody
SKU/Catalog Number
A01879-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human PDCD10 DyLight® 550 conjugated Antibody catalog # A01879-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human PDCD10 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01879-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PDCD10 recombinant protein (Position: M1-A212).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01879-Dyl550 is reactive to PDCD10 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
24.702kDa
Background of PDCD10
Programmed cell death protein 10 is a protein that in humans is encoded by the PDCD10 gene. This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01879-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For PDCD10 (Source: Uniprot.org, NCBI)
Gene Name
PDCD10
Full Name
Programmed cell death protein 10
Weight
24.702kDa
Superfamily
PDCD10 family
Alternative Names
Programmed cell death protein 10; Cerebral cavernous malformations 3 protein; TF-1 cell apoptosis-related protein 15; PDCD10; CCM3; TFAR15 PDCD10 CCM3, TFAR15 programmed cell death 10 programmed cell death protein 10|TF-1 cell apoptosis-related protein 15|apoptosis-related protein 15|cerebral cavernous malformations 3 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD10, check out the PDCD10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human PDCD10 DyLight® 550 conjugated Antibody (A01879-Dyl550)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Human PDCD10 DyLight® 550 conjugated Antibody
Question
Here is the WB image, lot number and protocol we used for platelet using anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-Human PDCD10 DyLight® 550 conjugated antibody (A01879-Dyl550) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
Will anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550 work for Flow Cytometry with platelet?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
According to the expression profile of platelet, PDCD10 is highly expressed in platelet. So, it is likely that anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550 will work for Flow Cytometry with platelet.
Boster Scientific Support
Answered: 2019-11-15
Question
Is this A01879-Dyl550 anti-Human PDCD10 DyLight® 550 conjugated antibody reactive to the isotypes of PDCD10?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
The immunogen of A01879-Dyl550 anti-Human PDCD10 DyLight® 550 conjugated antibody is E. coli-derived human PDCD10 recombinant protein (Position: M1-A212). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-24
Question
Does anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550 work on canine Flow Cytometry with skin urinary bladder?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
Our lab technicians have not validated anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Human PDCD10 DyLight® 550 conjugated antibody A01879-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine skin urinary bladder in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-17
Question
My question regarding product A01879-Dyl550, anti-Human PDCD10 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01879-Dyl550 anti-Human PDCD10 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-30
