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Product Info Summary
| SKU: | M00758-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SOX10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00758-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SOX10 Rabbit Monoclonal Antibody catalog # M00758-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00758-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GIB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00758-1 is reactive to SOX10 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
60-65 kDa
Calculated molecular weight
49911 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00758-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human A375 whole cell
ICC/IF: HT-29 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOX10 using anti-SOX10 antibody (M00758-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX10 antigen affinity purified monoclonal antibody (Catalog # M00758-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX10 at approximately 60-65 kDa. The expected band size for SOX10 is at 50 kDa.
Click image to see more details
Immunofluorescent analysis of HT-29 cells, using SOX10 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SOX10 (Source: Uniprot.org, NCBI)
Gene Name
SOX10
Full Name
Transcription factor SOX-10
Weight
49911 MW
Alternative Names
Transcription factor SOX-10;SOX10; SOX10 DOM, PCWH, WS2E, WS4, WS4C SRY-box transcription factor 10 transcription factor SOX-10|SRY (sex determining region Y)-box 10|SRY-box 10|SRY-related HMG-box gene 10|dominant megacolon, mouse, human homolog of
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX10, check out the SOX10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX10 Rabbit Monoclonal Antibody (M00758-1)
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Customer Q&As
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15 Customer Q&As for Anti-SOX10 Rabbit Monoclonal Antibody
Question
We ordered your anti-SOX10 Rabbit Monoclonal antibody for WB on placenta skin in the past. I am using human, and We are going to use the antibody for IF next. I was wanting to use examining placenta skin as well as small intestine in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
I took a look at the website and datasheets of our anti-SOX10 Rabbit Monoclonal antibody and it appears that M00758-1 has been validated on human in both WB and IF. Thus M00758-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-29
Question
Can you help my question with product M00758-1, anti-SOX10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Miller
Verified customer
Asked: 2020-03-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00758-1 anti-SOX10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-SOX10 Rabbit Monoclonal antibody (M00758-1)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do provide the blocking peptide for product anti-SOX10 Rabbit Monoclonal antibody (M00758-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-13
Question
We have observed staining in rat inferior olivary complex. Are there any suggestions? Is anti-SOX10 Rabbit Monoclonal antibody supposed to stain inferior olivary complex positively?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
From what I have seen in literature inferior olivary complex does express SOX10. From what I have seen in Uniprot.org, SOX10 is expressed in inferior olivary complex, small intestine, placenta skin, among other tissues. Regarding which tissues have SOX10 expression, here are a few articles citing expression in various tissues:
Placenta, and Skin, Pubmed ID: 15489334
Small intestine, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-08
Question
Will anti-SOX10 Rabbit Monoclonal antibody M00758-1 work for IF with small intestine?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
According to the expression profile of small intestine, SOX10 is highly expressed in small intestine. So, it is likely that anti-SOX10 Rabbit Monoclonal antibody M00758-1 will work for IF with small intestine.
Boster Scientific Support
Answered: 2019-12-26
Question
Does M00758-1 anti-SOX10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
As indicated on the product datasheet, M00758-1 anti-SOX10 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-SOX10 Rabbit Monoclonal antibody M00758-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-SOX10 Rabbit Monoclonal antibody (M00758-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
See attached the WB image, lot number and protocol we used for small intestine using anti-SOX10 Rabbit Monoclonal antibody M00758-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
I see that the anti-SOX10 Rabbit Monoclonal antibody M00758-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-04
Question
Is this M00758-1 anti-SOX10 Rabbit Monoclonal antibody reactive to the isotypes of SOX10?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The immunogen of M00758-1 anti-SOX10 Rabbit Monoclonal antibody is A synthesized peptide derived from human SOX10 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-28
Question
Do you have a BSA free version of anti-SOX10 Rabbit Monoclonal antibody M00758-1 available?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SOX10 Rabbit Monoclonal antibody M00758-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-22
Question
Our lab were content with the WB result of your anti-SOX10 Rabbit Monoclonal antibody. However we have been able to see positive staining in inferior olivary complex cytoplasm. using this antibody. Is that expected? Could you tell me where is SOX10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
Based on literature, inferior olivary complex does express SOX10. Generally SOX10 expresses in cytoplasm. Regarding which tissues have SOX10 expression, here are a few articles citing expression in various tissues:
Placenta, and Skin, Pubmed ID: 15489334
Small intestine, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-06-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine using anti-SOX10 Rabbit Monoclonal antibody M00758-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-18
Question
We want to test anti-SOX10 Rabbit Monoclonal antibody M00758-1 on human small intestine for research purposes, then I may be interested in using anti-SOX10 Rabbit Monoclonal antibody M00758-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
The products we sell, including anti-SOX10 Rabbit Monoclonal antibody M00758-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-13
Question
I was wanting to use your anti-SOX10 Rabbit Monoclonal antibody for IF for human small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine identification?
K. Wu
Verified customer
Asked: 2014-10-07
Answer
As indicated on the product datasheet, M00758-1 anti-SOX10 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-07
