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Product Info Summary
| SKU: | PB9397 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Smad4 Antibody Picoband®
SKU/Catalog Number
PB9397
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smad4 Antibody Picoband® catalog # PB9397. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9397)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9397 is reactive to SMAD4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
60439 MW
Background of SMAD4
SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFβ protein superfamily. Hahn et al. (1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al. (1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9397 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, Rat Skeletal Muscle Tissue, Mouse Skeletal Muscle Tissue, U87 Whole Cell, Human Placenta Tissue, HT1080 Whole Cell, HELA Whole Cell, NEURO Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-SMAD4 Picoband antibody, PB9397, Western blotting
All lanes: Anti SMAD4 (PB9397) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: Human Placenta Tissue Lysate at 50ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Lane 8: HELA Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Protein Target Info & Infographic
Gene/Protein Information For SMAD4 (Source: Uniprot.org, NCBI)
Gene Name
SMAD4
Full Name
Mothers against decapentaplegic homolog 4
Weight
60439 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4; SMAD4 DPC4, JIP, MADH4, MYHRS SMAD family member 4 mothers against decapentaplegic homolog 4|MAD homolog 4|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|deletion target in pancreatic carcinoma 4|mothers against decapentaplegic, Drosophila, homolog of, 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD4, check out the SMAD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad4 Antibody Picoband® (PB9397)
Hello CJ!
PB9397 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu,R.,Zhou,X., Li,Y.,Ren,L.,& He,X. (2021).MicroRNA‑181a promotes epithelial‑mesenchymal transition in esophageal squamous cell carcinoma via the TGF‑β/Smad pathway.Molecular Medicine Reports,23,316. https://doi.org/10.3892/mmr.2021.11955
Species: Human
PB9397 usage in article: APP:WB, SAMPLE:TE-1 CELL, DILUTION:1:100-1:400
TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhibiting PIK3R3 expression
Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
Molecular responses of radiation-induced liver damage in rats
Chen Wh, Kong Xy, Wan R, Xiao Cs, Li L, Wang Zy, Lin N, Wang Hm. Chin J Integr Med. 2012 May;18(5):378-84. Doi: 10.1007/S11655-012-1086-Y. Epub 2012 May 2. Effects Of Huogu I Formula (I) On Correlated Factors Of Bone Regeneration In Chickens With ...
Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. 2014 Jun;25(6):675-82. Doi: 10.1016/J.Jnutbio.2014.02.009. Epub 2014 Mar 19. Quercetin Prevents Ethanol-Induced Iron Overload By Regulating Hepcidin Through The Bmp6/S...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Smad4 Antibody Picoband®
Question
Would PB9397 anti-Smad4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can see on the product datasheet, PB9397 anti-Smad4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-06
Question
I am looking for to test anti-Smad4 antibody PB9397 on human kidney for research purposes, then I may be interested in using anti-Smad4 antibody PB9397 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The products we sell, including anti-Smad4 antibody PB9397, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-27
Question
Is this PB9397 anti-Smad4 antibody reactive to the isotypes of SMAD4?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The immunogen of PB9397 anti-Smad4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4 (526-552aa EIHLHRALQLLDEVLHTMPIADPQPLD), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-30
Question
We have seen staining in rat fetal brain. Are there any suggestions? Is anti-Smad4 antibody supposed to stain fetal brain positively?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
From what I have seen in literature fetal brain does express SMAD4. From what I have seen in Uniprot.org, SMAD4 is expressed in kidney, fetal brain, placenta, muscle, liver, among other tissues. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2019-11-14
Question
My question regards using your anti-Smad4 antibody for atrioventricular valve formation studies. Has this antibody been tested with western blotting on neuro whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Thank you for your inquiry. This PB9397 anti-Smad4 antibody is tested on rat brain tissue, tissue lysate, mouse brain, skeletal muscle tissue, u87 whole cell lysate, human placenta tissue, ht1080 whole cell lysate, hela whole cell lysate, neuro whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-31
Question
Can you help my question with product PB9397, anti-Smad4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9397 anti-Smad4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Smad4 antibody PB9397. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-24
Question
Is there a BSA free version of anti-Smad4 antibody PB9397 available?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Smad4 antibody PB9397 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-03
Question
Will anti-Smad4 antibody PB9397 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
According to the expression profile of kidney, SMAD4 is highly expressed in kidney. So, it is likely that anti-Smad4 antibody PB9397 will work for WB with kidney.
Boster Scientific Support
Answered: 2018-07-26
Question
We are currently using anti-Smad4 antibody PB9397 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
The anti-Smad4 antibody (PB9397) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-28
Question
I see that the anti-Smad4 antibody PB9397 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-05
Question
Is a blocking peptide available for product anti-Smad4 antibody (PB9397)?
W. Krishna
Verified customer
Asked: 2015-09-21
Answer
We do provide the blocking peptide for product anti-Smad4 antibody (PB9397). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-21
Question
I was wanting to use your anti-Smad4 antibody for WB for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
T. Jones
Verified customer
Asked: 2015-06-19
Answer
You can see on the product datasheet, PB9397 anti-Smad4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-19
Question
I have attached the WB image, lot number and protocol we used for kidney using anti-Smad4 antibody PB9397. Please let me know if you require anything else.
J. Kulkarni
Verified customer
Asked: 2015-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-21
Question
My colleagues were happy with the WB result of your anti-Smad4 antibody. However we have seen positive staining in placenta cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD4 supposed to be expressed?
S. Anderson
Verified customer
Asked: 2014-01-24
Answer
Based on literature, placenta does express SMAD4. Generally SMAD4 expresses in cytoplasm. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2014-01-24
