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Product Info Summary
| SKU: | A12912 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-PIGH Antibody
SKU/Catalog Number
A12912
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PIGH Antibody (Catalog # A12912). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIGH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12912)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human PIGH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12912 is reactive to PIGH in Human, Mouse
Reconstitution
Calculated molecular weight
21081 MW
Background of PIGH
Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
Kamitani T., J. Biol. Chem. 268:20733-20736(1993).
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12912 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using PIGH antibody A12912.
Protein Target Info & Infographic
Gene/Protein Information For PIGH (Source: Uniprot.org, NCBI)
Gene Name
PIGH
Full Name
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H
Weight
21081 MW
Superfamily
PIGH family
Alternative Names
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H;2.4.1.198;Phosphatidylinositol-glycan biosynthesis class H protein;PIG-H;PIGH; PIGH GPI-H phosphatidylinositol glycan anchor biosynthesis class H phosphatidylinositol N-acetylglucosaminyltransferase subunit H|phosphatidylinositol-glycan biosynthesis, class H protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIGH, check out the PIGH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIGH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIGH Antibody (A12912)
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No publications found for A12912
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PIGH Antibody
Question
Is this A12912 anti-PIGH antibody reactive to the isotypes of PIGH?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The immunogen of A12912 anti-PIGH antibody is Synthesized peptide derived from C-terminal of human PIGH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-16
Question
Is a blocking peptide available for product anti-PIGH antibody (A12912)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We do provide the blocking peptide for product anti-PIGH antibody (A12912). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-PIGH antibody A12912 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-06
Question
We are currently using anti-PIGH antibody A12912 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
N. Li
Verified customer
Asked: 2019-02-08
Answer
The anti-PIGH antibody (A12912) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-08
Question
Does anti-PIGH antibody A12912 work on bovine IHC with placenta?
D. Wu
Verified customer
Asked: 2015-09-18
Answer
Our lab technicians have not tested anti-PIGH antibody A12912 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PIGH antibody A12912 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-18
Question
Will A12912 anti-PIGH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Walker
Verified customer
Asked: 2014-07-01
Answer
As indicated on the product datasheet, A12912 anti-PIGH antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-01
