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Product Info Summary
| SKU: | A00059S204-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, ICC, WB |
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Product info
Product Name
Anti-Smad3 (phospho-S204) Antibody
SKU/Catalog Number
A00059S204-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Smad3 (phospho-S204) Antibody catalog # A00059S204-1. Tested in WB,IHC,ICC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad3 (phospho-S204) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00059S204-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 204.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00059S204-1 is reactive to SMAD3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
48081 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00059S204-1 is guaranteed for IP, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of SMAD3 using Anti-Smad3 (phospho-S204) Antibody (A00059S204-1).
Immunohistochemistry (IHC) analyzes of p-Smad3 (S204) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 3. Immunohistochemistry validation of SMAD3 using Anti-Smad3 (phospho-S204) Antibody (A00059S204-1).
Immunohistochemistry (IHC) analyzes of p-Smad3 (S204) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-Smad3 (phospho-S204) Antibody A00059S204-1
Western blot (WB) analysis of p-Smad3 (S204) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate treated with UV(4h)
Lane2:sp2/0 cell lysate treated with UV(4h)
Lane3:H9C2 cell lysate treated with UV(4h)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SMAD3 (Source: Uniprot.org, NCBI)
Gene Name
SMAD3
Full Name
Mothers against decapentaplegic homolog 3
Weight
48081 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3;SMAD3;MADH3; SMAD3 HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3 SMAD family member 3 mothers against decapentaplegic homolog 3|MAD homolog 3|MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|hMAD-3|hSMAD3|mad homolog JV15-2|mad protein homolog|mad3|mothers against DPP homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD3, check out the SMAD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad3 (phospho-S204) Antibody (A00059S204-1)
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