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Product Info Summary
| SKU: | A11935-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SLC35D1 Antibody Picoband®
SKU/Catalog Number
A11935-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC35D1 Antibody Picoband® catalog # A11935-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC35D1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11935-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC35D1 recombinant protein (Position: M1-V355).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11935-2 is reactive to SLC35D1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.24kDa
Background of SLC35D1
Glycosylation of cellular glycoconjugates occurs in the endoplasmic reticulum (ER) and Golgi compartment, and requires transport of nucleotide sugars from the cytosol into the lumen of the ER and Golgi by specific transporters. The protein encoded by this gene resides in the ER, and transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm to the ER lumen. It may participate in glucuronidation and/or chondroitin sulfate biosynthesis. Mutations in this gene are associated with Schneckenbecken dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11935-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SIHA whole cell, human Daudi whole cell, mouse thymus tissue
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC35D1 using anti-SLC35D1 antibody (A11935-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35D1 antigen affinity purified polyclonal antibody (Catalog # A11935-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35D1 at approximately 39 kDa. The expected band size for SLC35D1 is at 39 kDa.
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Figure 2. Flow Cytometry analysis of U87 cells using anti-SLC35D1 antibody (A11935-2).
Overlay histogram showing U87 cells stained with A11935-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC35D1 Antibody (A11935-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SLC35D1 (Source: Uniprot.org, NCBI)
Gene Name
SLC35D1
Full Name
UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter
Weight
39.24kDa
Superfamily
TPT transporter family
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 6; Glucose transporter type 6; GLUT-6; Glucose transporter type 9; GLUT-9; SLC2A6; GLUT9 SLC35D1 SHNKND, UGTREL7 solute carrier family 35 member D1 UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter|UDP-GlcA/UDP-GalNAc transporter|UDP-galactose transporter-related 7|UDP-galactose transporter-related protein 7|solute carrier family 35 (UDP-GlcA/UDP-GalNAc transporter), member D1|solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC35D1, check out the SLC35D1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC35D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC35D1 Antibody Picoband® (A11935-2)
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