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Product Info Summary
| SKU: | A00616-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Desmoplakin/DSP Antibody Picoband®
View all Desmoplakin Antibodies
SKU/Catalog Number
A00616-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Desmoplakin/DSP Antibody Picoband® catalog # A00616-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Desmoplakin/DSP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00616-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Desmoplakin recombinant protein (Position: Q1810-A2092).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00616-1 is reactive to DSP in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
250-280 kDa
Calculated molecular weight
57371 MW
Background of Desmoplakin
Desmoplakin is a protein in humans that is encoded by the DSP gene. This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00616-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Desmoplakin using anti-Desmoplakin antibody (A00616-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoplakin antigen affinity purified polyclonal antibody (Catalog # A00616-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoplakin at approximately 250-280KD. The expected band size for Desmoplakin is at 332KD.
Protein Target Info & Infographic
Gene/Protein Information For DSP (Source: Uniprot.org, NCBI)
Gene Name
DSP
Full Name
Desmoplakin
Weight
57371 MW
Superfamily
plakin or cytolinker family
Alternative Names
Desmoplakin; DP; 250/210 kDa paraneoplastic pemphigus antigen; DSP DSP DCWHKTA, DP desmoplakin desmoplakin|250/210 kDa paraneoplastic pemphigus
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DSP, check out the DSP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DSP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Desmoplakin/DSP Antibody Picoband® (A00616-1)
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Customer Q&As
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17 Customer Q&As for Anti-Desmoplakin/DSP Antibody Picoband®
Question
My lab would like using your anti-Desmoplakin/DSP antibody for protein localization to adherens junction studies. Has this antibody been tested with western blotting on human hepg2 whole cell lysates? We would like to see some validation images before ordering.
S. Johnson
Verified customer
Asked: 2020-04-24
Answer
Thank you for your inquiry. This A00616-1 anti-Desmoplakin/DSP antibody is tested on human hepg2 whole cell lysates. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for zone of skin using anti-Desmoplakin/DSP antibody A00616-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-Desmoplakin/DSP antibody A00616-1 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-Desmoplakin/DSP antibody (A00616-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
I see that the anti-Desmoplakin/DSP antibody A00616-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-08
Question
My colleagues were content with the WB result of your anti-Desmoplakin/DSP antibody. However we have been able to see positive staining in liver cell junction using this antibody. Is that expected? Could you tell me where is DSP supposed to be expressed?
S. Miller
Verified customer
Asked: 2019-10-18
Answer
From literature, liver does express DSP. Generally DSP expresses in cell junction, desmosome. Regarding which tissues have DSP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 1689290, 1731325
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 10594734
Boster Scientific Support
Answered: 2019-10-18
Question
Is there a BSA free version of anti-Desmoplakin/DSP antibody A00616-1 available?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Desmoplakin/DSP antibody A00616-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-26
Question
Is this A00616-1 anti-Desmoplakin/DSP antibody reactive to the isotypes of DSP?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The immunogen of A00616-1 anti-Desmoplakin/DSP antibody is E. coli-derived human Desmoplakin recombinant protein (Position: Q1810-A2092). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-17
Question
Would anti-Desmoplakin/DSP antibody A00616-1 work on canine WB with liver?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Our lab technicians have not tested anti-Desmoplakin/DSP antibody A00616-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Desmoplakin/DSP antibody A00616-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-22
Question
See attached the WB image, lot number and protocol we used for zone of skin using anti-Desmoplakin/DSP antibody A00616-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-18
Question
I was wanting to use your anti-Desmoplakin/DSP antibody for ELISA for rat zone of skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat zone of skin identification?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
You can see on the product datasheet, A00616-1 anti-Desmoplakin/DSP antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat zone of skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-14
Question
We have been able to see staining in mouse cervix carcinoma. Any tips? Is anti-Desmoplakin/DSP antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
From what I have seen in literature cervix carcinoma does express DSP. From what I have seen in Uniprot.org, DSP is expressed in zone of skin, foreskin, skin, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have DSP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 1689290, 1731325
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 10594734
Boster Scientific Support
Answered: 2018-06-05
Question
Is a blocking peptide available for product anti-Desmoplakin/DSP antibody (A00616-1)?
G. Krishna
Verified customer
Asked: 2018-06-01
Answer
We do provide the blocking peptide for product anti-Desmoplakin/DSP antibody (A00616-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-01
Question
My question regarding product A00616-1, anti-Desmoplakin/DSP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00616-1 anti-Desmoplakin/DSP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-23
Question
Will anti-Desmoplakin/DSP antibody A00616-1 work for ELISA with zone of skin?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
According to the expression profile of zone of skin, DSP is highly expressed in zone of skin. So, it is likely that anti-Desmoplakin/DSP antibody A00616-1 will work for ELISA with zone of skin.
Boster Scientific Support
Answered: 2017-11-21
Question
We ordered your anti-Desmoplakin/DSP antibody for WB on leukemic t-cell last year. I am using mouse, and We want to use the antibody for ELISA next. I was wanting to use examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
I viewed the website and datasheets of our anti-Desmoplakin/DSP antibody and I see that A00616-1 has been tested on mouse in both WB and ELISA. Thus A00616-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-08-18
Question
Would A00616-1 anti-Desmoplakin/DSP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Thomas
Verified customer
Asked: 2015-04-29
Answer
You can see on the product datasheet, A00616-1 anti-Desmoplakin/DSP antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-04-29
Question
I would like to test anti-Desmoplakin/DSP antibody A00616-1 on rat zone of skin for research purposes, then I may be interested in using anti-Desmoplakin/DSP antibody A00616-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Miller
Verified customer
Asked: 2013-10-30
Answer
The products we sell, including anti-Desmoplakin/DSP antibody A00616-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-30
