Anti-SLC27A5/Fatp5 Antibody

Product Name

Anti-SLC27A5/Fatp5 Antibody

View all FATP5/SLC27A5 Antibodies

SKU/Catalog Number

A09287

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-SLC27A5/Fatp5 Antibody (Catalog # A09287). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SLC27A5/Fatp5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09287)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SLC27A5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09287 is reactive to SLC27A5 in Human

Reconstitution

Calculated molecular weight

75385 MW

Background of FATP5/SLC27A5

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.

Steinberg S.J., Mol. Genet. Metab. 68:32-42(1999).
Steinberg S.J., J. Biol. Chem. 275:15605-15608(2000).
Mihalik S.J., J. Biol. Chem. 277:24771-24779(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A09287 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For SLC27A5 (Source: Uniprot.org, NCBI)

Alternative Names

Bile acyl-CoA synthetase;BACS;6.2.1.7;Bile acid-CoA ligase;BA-CoA ligase;BAL;Cholate--CoA ligase;Fatty acid transport protein 5;FATP-5;Fatty-acid-coenzyme A ligase, very long-chain 3;Solute carrier family 27 member 5;Very long-chain acyl-CoA synthetase homolog 2;VLCS-H2;VLCSH2;Very long-chain acyl-CoA synthetase-related protein;VLACS-related;VLACSR;SLC27A5;ACSB, ACSVL6, FACVL3, FATP5; SLC27A5 ACSB, ACSVL6, BACS, BAL, FACVL3, FATP-5, FATP5, VLACSR, VLCS-H2, VLCSH2 solute carrier family 27 member 5 bile acyl-CoA synthetase|BA-CoA ligase|VLACS-related|bile acid-CoA ligase|cholate--CoA ligase|fatty acid transport protein 5|fatty-acid-Coenzyme A ligase, very long-chain 3|solute carrier family 27 (fatty acid transporter), member 5|very long-chain acyl-CoA synthetase homolog 2|very long-chain acyl-CoA synthetase-related protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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