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Product Info Summary
| SKU: | A30575 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-SLC27A5/Fatp5 Antibody
View all FATP5/SLC27A5 Antibodies
SKU/Catalog Number
A30575
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SLC27A5/Fatp5 Antibody (Catalog# A30575). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC27A5/Fatp5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30575)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SLC27A5. Immunogen sequence location: 481-530
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30575 is reactive to SLC27A5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
75385 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30575 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HepG2 cells, using SLC27A5 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using SLC27A5 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using SLC27A5 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SLC27A5 (Source: Uniprot.org, NCBI)
Gene Name
SLC27A5
Full Name
Bile acyl-CoA synthetase
Weight
75385 MW
Superfamily
ATP-dependent AMP-binding enzyme family
Alternative Names
SLC27A5;ACSB;ACSVL6;FACVL3;FATP5;Bile acyl-CoA synthetase;BACS;Bile acid-CoA ligase;BA-CoA ligase;BAL;Cholate--CoA ligase;Fatty acid transport protein 5;FATP-5;Fatty-acid-coenzyme A ligase; very long-chain 3;Solute carrier fam SLC27A5 ACSB, ACSVL6, BACS, BAL, FACVL3, FATP-5, FATP5, VLACSR, VLCS-H2, VLCSH2 solute carrier family 27 member 5 bile acyl-CoA synthetase|BA-CoA ligase|VLACS-related|bile acid-CoA ligase|cholate--CoA ligase|fatty acid transport protein 5|fatty-acid-Coenzyme A ligase, very long-chain 3|solute carrier family 27 (fatty acid transporter), member 5|very long-chain acyl-CoA synthetase homolog 2|very long-chain acyl-CoA synthetase-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC27A5, check out the SLC27A5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC27A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC27A5/Fatp5 Antibody (A30575)
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No publications found for A30575
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Customer Q&As
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6 Customer Q&As for Anti-SLC27A5/Fatp5 Antibody
Question
I see that the anti-SLC27A5/Fatp5 antibody A30575 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-20
Question
Is this A30575 anti-SLC27A5/Fatp5 antibody reactive to the isotypes of SLC27A5?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The immunogen of A30575 anti-SLC27A5/Fatp5 antibody is A peptide derived from human SLC27A5. Immunogen sequence location: 481-530. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-SLC27A5/Fatp5 antibody A30575 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The anti-SLC27A5/Fatp5 antibody (A30575) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-31
Question
My lab would like to test anti-SLC27A5/Fatp5 antibody A30575 on human right lobe of liver for research purposes, then I may be interested in using anti-SLC27A5/Fatp5 antibody A30575 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The products we sell, including anti-SLC27A5/Fatp5 antibody A30575, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-SLC27A5/Fatp5 antibody A30575. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-06
Question
My question regarding product A30575, anti-SLC27A5/Fatp5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30575 anti-SLC27A5/Fatp5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-15
