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Product Info Summary
| SKU: | A01061-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SIRT3 Antibody Picoband®
View all Sirtuin 3/SIRT3 Antibodies
SKU/Catalog Number
A01061-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIRT3 Antibody Picoband® catalog # A01061-3. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SIRT3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01061-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SIRT3, which shares 80% amino acid (aa) sequence identity with mouse and rat SIRT3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01061-3 is reactive to SIRT3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
54434 MW
Background of Sirtuin 3/SIRT3
NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01061-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
Positive Control
WB: human HepG2 whole cell, human Hacat whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SIRT3 using anti-SIRT3 antibody (A01061-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # A01061-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 43 kDa. The expected band size for SIRT3 is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SIRT3 (Source: Uniprot.org, NCBI)
Gene Name
SIRT3
Full Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Weight
54434 MW
Superfamily
sirtuin family
Alternative Names
Serine/threonine-protein kinase Chk1;2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1;CHEK1;CHK1; SIRT3 SIR2L3 sirtuin 3 NAD-dependent protein deacetylase sirtuin-3, mitochondrial|NAD-dependent deacetylase sirtuin-3, mitochondrial|SIR2-like protein 3|mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase|regulatory protein SIR2 homolog 3|silent mating type information regulation 2, S.cerevisiae, homolog 3|sir2-like 3|sirtuin type 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT3, check out the SIRT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT3 Antibody Picoband® (A01061-3)
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