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Product Info Summary
| SKU: | A05549 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Sipa1 Antibody
SKU/Catalog Number
A05549
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Sipa1 Antibody (Catalog # A05549). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Sipa1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05549)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05549 is reactive to SIPA1 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
112.149kDa
Background of SIPA1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05549 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000
IHC: 1.25-2.5 µg/ml
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
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Affinity purified anti-Sipatissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Figure 2. Western blot analysis of SIPA1 using anti-SIPA1 antibody (A05549).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIPA1 antigen affinity purified polyclonal antibody (Catalog # A05549) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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Figure 3. IHC analysis of SIPA1 using anti-SIPA1 antibody (A05549).
SIPA1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SIPA1 Antibody (A05549) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of SIPA1 using anti-SIPA1 antibody (A05549).
SIPA1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SIPA1 Antibody (A05549) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SIPA1 (Source: Uniprot.org, NCBI)
Gene Name
SIPA1
Full Name
Signal-induced proliferation-associated protein 1
Weight
112.149kDa
Alternative Names
Sipa1, Sipa-1, Sipa 1, GTPase activating protein Spa 1, p130 SPA-1, Signal induced proliferation associated 1, SPA1 SIPA1 SPA1 signal-induced proliferation-associated 1 signal-induced proliferation-associated protein 1|GTPase-activating protein Spa-1|p130 SPA-1|signal-induced proliferation-associated gene 1|sipa-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIPA1, check out the SIPA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sipa1 Antibody (A05549)
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Customer Q&As
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4 Customer Q&As for Anti-Sipa1 Antibody
Question
Would anti-Sipa1 antibody A05549 work for WB with peripheral blood lymphocyte?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
According to the expression profile of peripheral blood lymphocyte, SIPA1 is highly expressed in peripheral blood lymphocyte. So, it is likely that anti-Sipa1 antibody A05549 will work for WB with peripheral blood lymphocyte.
Boster Scientific Support
Answered: 2019-11-14
Question
Would anti-Sipa1 antibody A05549 work on primate IHC with peripheral blood lymphocyte?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
Our lab technicians have not validated anti-Sipa1 antibody A05549 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Sipa1 antibody A05549 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate peripheral blood lymphocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-29
Question
We are currently using anti-Sipa1 antibody A05549 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
T. Bhatt
Verified customer
Asked: 2016-11-29
Answer
The anti-Sipa1 antibody (A05549) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-29
Question
I see that the anti-Sipa1 antibody A05549 works with WB, what is the protocol used to produce the result images on the product page?
B. Thomas
Verified customer
Asked: 2015-12-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-22
