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Product Info Summary
| SKU: | PB9391 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-SHC/SHC1 Antibody Picoband®
SKU/Catalog Number
PB9391
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHC/SHC1 Antibody Picoband® catalog # PB9391. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHC/SHC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9391)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9391 is reactive to SHC1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa, 52 kDa, 66 kDa
Calculated molecular weight
62822 MW
Background of SHC1
SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9391 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human K562 whole cell, human THP-1 whole cell, rat C6 whole cell, mouse thymus tissue, mouse RAW2467 whole cell, mouse NIH3T3 whole cell
IHC: Mouse Brain tissue, Rat Brain tissue, Human Lung Cancer tissue
IHC-F: Human Placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SHC using anti-SHC antibody (PB9391).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysate,
Lane 2: human Hela whole cell lysate,
Lane 3: human HepG2 whole cell lysate,
Lane 4: human Jurkat whole cell lysate,
Lane 5: human K562 whole cell lysate,
Lane 6: human THP-1 whole cell lysate,
Lane 7: rat C6 whole cell lysate,
Lane 8: mouse thymus tissue lysate,
Lane 9: mouse RAW246.7 whole cell lysate,
Lane 10: mouse NIH3T3 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PB9391) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
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Figure 2. IHC analysis of SHC1 using anti-SHC1 antibody (PB9391).
SHC1 was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SHC1 Antibody (PB9391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SHC1 using anti-SHC1 antibody (PB9391).
SHC1 was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SHC1 Antibody (PB9391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of SHC1 using anti-SHC1 antibody (PB9391).
SHC1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SHC1 Antibody (PB9391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of SHC1 using anti-SHC1 antibody (PB9391).
SHC1 was detected in frozen section of Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SHC1 Antibody (PB9391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SHC1 (Source: Uniprot.org, NCBI)
Gene Name
SHC1
Full Name
SHC-transforming protein 1
Weight
62822 MW
Alternative Names
SHC-transforming protein 1;SHC-transforming protein 3;SHC-transforming protein A;Src homology 2 domain-containing-transforming protein C1;SH2 domain protein C1;SHC1;SHC, SHCA; SHC1 SHC, SHCA SHC adaptor protein 1 SHC-transforming protein 1|SH2 domain protein C1|SHC (Src homology 2 domain containing) transforming protein 1|SHC-transforming protein 3|SHC-transforming protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHC1, check out the SHC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHC/SHC1 Antibody Picoband® (PB9391)
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Customer Q&As
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2 Customer Q&As for Anti-SHC/SHC1 Antibody Picoband®
Question
We are currently using anti-SHC/SHC1 antibody PB9391 for mouse tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
A. Kulkarni
Verified customer
Asked: 2019-04-25
Answer
The anti-SHC/SHC1 antibody (PB9391) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-25
Question
I am interested in your PB9391 and PA1897 SHC1 antibody, have you also saw 3 isoforms in any of your tested samples?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Please see the updated image of PB9391 and PA1897 on the product pages. https://www.bosterbio.com/anti-shc-picoband-trade-antibody-pb9391-boster.html?options=cart https://www.bosterbio.com/anti-shc-antibody-pa1897-boster.html?options=cart Both PB9391 and PA1897 are able to detect 3 isoforms.
Boster Scientific Support
Answered: 2017-09-13
