Anti-SHC/SHC1 Antibody Picoband®

Product Name

Anti-SHC/SHC1 Antibody Picoband®

View all SHC1 Antibodies

SKU/Catalog Number

PA1897

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SHC/SHC1 Antibody catalog # PA1897. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SHC/SHC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1897)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1897 is reactive to SHC1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

46 kDa, 52 kDa, 66 kDa

Calculated molecular weight

62822 MW

Background of SHC1

SHC1 (SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al. (1994) assigned the SHC1 gene to 1q21. Yulug et al. (1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence (SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al. (1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85% identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel (2002) concluded that there is an important functional relationship between forkhead proteins (e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1897 is guaranteed for IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse thymus tissue, mouse RAW2467 whole cell, mouse NIH3T3 whole cell
IHC: Rat Brain Tissue, Human Lung Cancer Tissue
IHC-F: Rat Intestine Tissue, Rat Brain Tissue
ICC: A549 Cell

Validation Images & Assay Conditions

Gene/Protein Information For SHC1 (Source: Uniprot.org, NCBI)

Alternative Names

SHC-transforming protein 1;SHC-transforming protein 3;SHC-transforming protein A;Src homology 2 domain-containing-transforming protein C1;SH2 domain protein C1;SHC1;SHC, SHCA; SHC1 SHC, SHCA SHC adaptor protein 1 SHC-transforming protein 1|SH2 domain protein C1|SHC (Src homology 2 domain containing) transforming protein 1|SHC-transforming protein 3|SHC-transforming protein A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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