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Product Info Summary
| SKU: | EK1633 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Dermatopontin/DPT ELISA Kit PicoKine®
View all Dermatopontin ELISA kits
SKU/Catalog Number
EK1633
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Dermatopontin/DPT ELISA Kit PicoKine® (96 Tests). Quantitate Human DPT in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Dermatopontin/DPT ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1633)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q19-V201
Sensitivity
<10 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1633 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1633 is reactive to DPT in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1633 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dermatopontin
Dermatopontin is a protein that in humans is encoded by the DPT gene. It’s an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1633-CAP | Anti-Human DPT Pre-coated 96-well strip microplate | 1 |
| EK1633-ST | Human DPT Standard | 2 vials, 10 ng/tube |
| EK1633-DA | Human DPT Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.022 | 0.058 | 0.117 | 0.266 | 0.591 | 1.157 | 1.685 | 2.062 |
Data Example Images
Click image to see more details
Human Dermatopontin/DPT PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Dermatopontin, Dilution ratio of 1:10, concentration in serum and plasma is 4-10 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Dermatopontin/DPT ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 100 | 977 | 2868 | 91 | 933 | 3012 |
| Standard deviation | 7.1 | 46.89 | 169.21 | 7.91 | 58.77 | 234.93 |
| CV (%) | 7.1% | 4.8% | 5.9% | 8.7% | 6.3% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Dermatopontin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 100 | 105 | 105 | 109 | 104 | 3.19 | 3% |
| Sample 2 | 977 | 1016 | 907 | 886 | 946 | 52.39 | 5.5% |
| Sample 3 | 2868 | 2711 | 2472 | 2675 | 2681 | 141.05 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For DPT (Source: Uniprot.Org, NCBI)
Gene Name
DPT
Full Name
Dermatopontin
Weight
24.005kDa
Superfamily
dermatopontin family
Alternative Names
Dermatopontin; Tyrosine-rich acidic matrix protein; TRAMP; DPT DPT TRAMP dermatopontin dermatopontin|tyrosine-rich acidic matrix protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DPT, check out the DPT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DPT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Dermatopontin/DPT ELISA Kit PicoKine® (EK1633)
Hello CJ!
EK1633 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human Dermatopontin/DPT ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-12-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-12-05
Question
Q: What is the optimal O.D. value for Dermatopontin ELISA kit? I used your Dermatopontin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Dermatopontin even though the O.D. values are not very high?
L. Mehta
Verified customer
Asked: 2020-03-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Dermatopontin ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of Dermatopontin that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2020-03-23
Question
Q: can I use citrate plasma as samples in Human Dermatopontin Picokine® ELISA Kit (Catalog # EK1633)?
P. Moore
Verified customer
Asked: 2020-02-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Dermatopontin causing disruption of its protein structure. Dermatopontin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Dermatopontin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-02-28
Question
Q: if the enzyme conjugated Dermatopontin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Dermatopontin antigen?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-10-23
Question
Q: how can I thaw whole blood sample for Dermatopontin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Dermatopontin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Sunder
Verified customer
Asked: 2019-01-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-22
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Anand
Verified customer
Asked: 2018-05-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-03
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Dermatopontin level in serum.
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-04-02
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Dermatopontin using the EK1633 Human Dermatopontin Picokine® ELISA Kit?
C. Babu
Verified customer
Asked: 2017-11-29
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2017-11-29
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Dermatopontin in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2016-08-25
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Dermatopontin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-08-25
