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- Table of Contents
Facts about SHC-transforming protein 1.
Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems.
Human | |
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Gene Name: | SHC1 |
Uniprot: | P29353 |
Entrez: | 6464 |
Belongs to: |
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No superfamily |
FLJ26504; p66; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC; SHC1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1
Mass (kDA):
62.822 kDA
Human | |
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Location: | 1q21.3 |
Sequence: | 1; NC_000001.11 (154962298..154974492, complement) |
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Cytoplasm.; [Isoform p46Shc]: Mitochondrion matrix. Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; [Isoform p66Shc]: Mitochondrion. In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
The SHC1 marker plays a crucial role in molecular biology. It allows you manipulate genetic material, such as DNA to look into a particular disease. Boster offers a wide array of technical resources, including blogs, disease information and web-based digital tools. In addition, it provides high-quality buffers for lysis that are designed for optimal results and minimize cross-linking intensity. This article will give you an overview of the products Boster Bio offers.
The primary-secondary-ABC system uses avidins that are conjugated to a signal molecule, such as Horseradish Peroxide (HRP), to locate proteins of interest. This is particularly useful for protein detection, as it has low background and high specificity. Other detection systems have been developed, which include organic polymers and polysaccharides. Boster Bio's Super Vision Detection kits are an example of these innovative systems.
If you're looking for the ideal antigen or detection kit take a look at the products offered by Boster Bio. Boster Bio is committed to addressing the needs of its clients, assisting them reach their research goals and keeping them on the cutting-edge of research. Boster Bio manufactures antibodies and ELISA kits in-house, ensuring that they're made to the highest quality and efficiency standards. Boster has the right kit to meet your needs, regardless of whether you are working in a research laboratory or clinical setting, or in a laboratory.
Boster Bio is a company that specializes in high-specificity, sensitive antibodies. Their techniques and technology have been refined over the past two decades. Their products have been referenced in more than 29,000 publications and have been tested for use in ELISA, IHC, and Flow Cytometry. Boster Bio also offers a diverse range of other products, including controls and lysates.
Many researchers make use of Protein A and G antibodies when immunoprecipitating. Protein A/G is particularly effective because it contains four Protein A antibody binding sites as well as two Protein G affinity beads. These antibodies are highly compatible with various subclasses. Both kinds of antibodies work with beads and can be used together.
Follow the steps in Method A to prepare the Sepharose beads made of protein A/G. First, you must prepare an agarose sample using SDS or lysis buffer. If you use SDS it will release non-covalently bound antibodies. Glycine buffer can be utilized to decrease the amount of protein that is eluted. The pH of this buffer ranges from 2.0 to 3.0. Glycine is an ideal buffer since it reduces the antibody-bead interactions. Glycine is also reusable. After elution, neutralize the sample with Tris buffer or PBS.
SureBeads are extremely efficient in the process of lysing samples. The lysate must be cleared prior to use. appropriate buffer is required. This can be accomplished by separating the sample with NuPAGE 4-12 bis-Tris gradient gel. After separation, the lysate must be transferred to a different tube. Before the final magnetization, it should be added to 40 ul of reduced Laemmli sample buffer. The sample should be incubated at 70°C for 10 mins, followed by 5 minutes at 95°C. It should be stored at -20C up to the point of use after clearing.
There are many benefits offered by protein A/G affinity beads. They enable an efficient lysate as they do not allow centrifugation to break weak antibody-antigen binding and result in the loss or damage of the target proteins. Because they are smaller than other beads, it is simpler to manually pipet solutions from them. To make the entire process easier microplates that have 96 wells are available.
Protein interactions can be discovered using Co-immunoprecipitation. It is similar to IP, except that it utilizes a target antigen to co-precipitate proteins that are associated with the antigen. The proteins are then verified to be functionally related to the antigen. This protocol is applicable to unmodified cells too.
Here are some suggestions from Boster Bio to optimize your SHC1 experiment. These are the best ways to determine the most effective markers for your SHC1 study. Nioite - These are Ben Ming, Li Huan and Gai Shan. Tie ni, Ben Ming, and Te Ni are all identified markers for the SHC1 marker. They are also known by the mineral 'tea tree' name and could influence test results.
PMID: 1623525 by Pelicci G., et al. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.
PMID: 9049300 by Migliaccio E., et al. Opposite effects of the p52shc/p46shc and p66shc splicing isoforms on the EGF receptor-MAP kinase-fos signalling pathway.